XFD350 TCO
Product key features
- Ex/Em: 343/441 nm
- Extinction coefficient: 19,000 cm-1M-1
- Reactive Group: TCO
- Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
- Moderate Brightness & pH Stability: Ideal for high-abundance targets and maintains consistent fluorescence across a broad pH range (4–11)
- High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity
Product description
XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.
XFD350 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.