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AAT Bioquest

XFD350 TCO

Product key features

  • Ex/Em: 343/441 nm
  • Extinction coefficient: 19,000 cm-1M-1
  • Reactive Group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
  • Moderate Brightness & pH Stability: Ideal for high-abundance targets and maintains consistent fluorescence across a broad pH range (4–11)
  • High Contrast: Minimizes spectral overlap with common fluorophores like fluorescein and XFD488, enhancing multiplexing and signal clarity

Product description

XFD350, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor® 350 (ThermoFisher). This blue-fluorescent dye exhibits strong absorption and moderate photostability, with consistent fluorescence across a broad pH range (pH 4-11). XFD350 is effectively excited by the 350 nm line of a UV laser, and its moderate brightness and stability make it particularly advantageous for direct imaging of high-abundance targets in both microscopy and flow cytometry applications.

XFD350 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Page updated on April 25, 2025

Ordering information

Price
Unit size
Catalog Number70006
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
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Physical properties

Molecular weight

622.78

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.25

Correction Factor (280 nm)

0.19

Extinction coefficient (cm -1 M -1)

19000

Excitation (nm)

343

Emission (nm)

441

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a trans‐cyclooctene (TCO)–dye conjugate and a tetrazine‐modified biomolecule. The TCO–dye adduct selectively and rapidly couples with the tetrazine moiety on the biomolecule to form a stable linkage. This bioorthogonal “click chemistry” reaction is fast, selective, and well‐suited for biocompatible labeling applications.
Gallery Image 2