XFD350 tyramide reagent

XFD350 Tyramide Reagent is a blue fluorescent dye with an excitation/emission of 343/441 nm that is used for flow cytometry and microscopy applications.

Tyramide Signal Amplification (TSA): Facilitates ultrasensitive detection of low-abundance targets, ideal for IHC and ICC applications
Dependable XFD350 Dye: Offers moderate photostability and consistent fluorescence across a wide pH range (4–11)

The chemical structure of XFD350 tyramide.

Protocol

SDS

Catalog	Size	Price	Quantity
11067	200 Slides	Price

Overview

XFD350 is manufactured by AAT Bioquest, and it has a similar chemical structure as Alexa Fluor® 350 (Alexa Fluor® is the trademark of Thermo Fisher). For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from the TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD350 tyramide contains the bright XFD350 dye that can be readily detected with the standard DAPI filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD350 tyramide reagent to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	231.241 µL	1.156 mL	2.312 mL	11.562 mL	23.124 mL
5 mM	46.248 µL	231.241 µL	462.481 µL	2.312 mL	4.625 mL
10 mM	23.124 µL	115.62 µL	231.241 µL	1.156 mL	2.312 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	432.45 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

High Resolution Fluorescent In Situ Hybridization in Drosophila Embryos and Tissues Using Tyramide Signal Amplification

Authors:

J, undefined and ura, A., Hu, J., Wilk, R., Krause, H. M.

Journal:

J Vis Exp (2017): se name="11070.enl" path="C:\Website\Referenc

Signal amplification in the detection of single-copy DNA and RNA by enzyme-catalyzed deposition (CARD) of the novel fluorescent reporter substrate Cy3.29-tyramide

Authors:

Schmidt, B. F., Chao, J., Zhu, Z., DeBiasio, R. L., Fisher, G.

Journal:

J Histochem Cytochem (1997): 365-73

Physical properties

Molecular weight	432.45
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

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Page updated on April 12, 2026