Name: XFD350-Wheat Germ Agglutinin (WGA) Conjugate
Brand: AAT Bioquest
SKU: 25495
Price: 111 USD
Availability: InStock

XFD350-Wheat Germ Agglutinin (WGA) Conjugate

XFD350, manufactured by AAT Bioquest, has a similar chemical structure to Alexa Fluor® 350, a trademark of Thermo Fisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD350 WGA conjugate, like its counterpart Alexa Fluor® 350 WGA conjugate, emits a bright blue fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25495	1 mg	Price

References

View all 50 references: Citation Explorer

Wheat Germ Agglutinin Affinity Chromatography Enrichment and Glyco-Proteomic Characterization of Tetrodotoxin-Binding Proteins from the Plasma of Cultured Tiger Pufferfish (Takifugu rubripes).

Authors:

Zhang, Yafei and Minami, Ryoma and Tatsuno, Ryohei and Gao, Wei and Ueno, Mikinori and Yamada, Akinori and Yoshida, Asami and Sedanza, Mary Grace and Arima, Kazunari and Takatani, Tomohiro and Yamaguchi, Kenichi and Oshima, Yuji and Arakawa, Osamu

Journal:

Bioscience, biotechnology, and biochemistry (2023)

Microscopic observation of human airway ciliary movement using wheat germ agglutinin.

Authors:

Nakamura, Ryosuke and Oyagi, Seiji and Katsuno, Tatsuya and Kishimoto, Yo and Omori, Koichi

Journal:

Methods in cell biology (2023): 33-43

Ultrasensitive Fluorescence Lateral Flow Assay for Simultaneous Detection of Pseudomonas aeruginosa and Salmonella typhimurium via Wheat Germ Agglutinin-Functionalized Magnetic Quantum Dot Nanoprobe.

Authors:

Tu, Zhijie and Yang, Xingsheng and Dong, Hao and Yu, Qing and Zheng, Shuai and Cheng, Xiaodan and Wang, Chongwen and Rong, Zhen and Wang, Shengqi

Journal:

Biosensors (2022)

Universal and ultrasensitive detection of foodborne bacteria on a lateral flow assay strip by using wheat germ agglutinin-modified magnetic SERS nanotags.

Authors:

Tu, Zhijie and Cheng, Siyun and Dong, Hao and Wang, Wenqi and Yang, Xingsheng and Gu, Bing and Wang, Shengqi and Wang, Chongwen

Journal:

RSC advances (2022): 27344-27354

Biogenesis of Exosomes Laden with Metallic Silver-Copper Nanoparticles Liaised by Wheat Germ Agglutinin for Targeted Delivery of Therapeutics to Breast Cancer.

Authors:

Ashraf, Sarmadia and Qadri, Shahnaz and Akbar, Shayista and Parray, Aijaz and Haik, Yousef

Journal:

Advanced biology (2022): e2200005

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.25
Correction factor (280 nm)	0.19
Extinction coefficient (cm ^-1 M ^-1)	19000
Excitation (nm)	343
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	DAPI filter set
Emission	DAPI filter set
Recommended plate	Black wall/clear bottom

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Page updated on May 4, 2026