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AAT Bioquest

XFD405 alkyne

Product key features

  • Ex/Em: 401/421 nm
  • Extinction coefficient: 35,000 cm-1M-1
  • Reactive Group: Alkyne
  • Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
  • Consistent Fluorescence: pH-insensitive from pH 4 to 10 and minimal quenching when conjugated to proteins
  • High Photostability: Ensures reliable performance for multicolor flow cytometry and advanced imaging applications like STORM

Product description

XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor™ 405 (ThermoFisher). This dye is water-soluble and optimized for excitation by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability.

The alkyne derivative of XFD405 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.

Spectrum

Page updated on April 15, 2025

Ordering information

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Catalog Number70014
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

666.69

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.70

Extinction coefficient (cm -1 M -1)

35,000

Excitation (nm)

401

Emission (nm)

421

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
Product Image
Gallery Image 1
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an alkyne‐functionalized dye and an azide‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions, tolerates a wide range of functional groups, and is frequently used to label proteins, nucleic acids, and other biomolecules for imaging, proteomics, and high‐throughput assays.