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AAT Bioquest

XFD405 amine

Product key features

  • Ex/Em: 401/421 nm
  • Extinction coefficient: 35,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • Consistent Fluorescence: pH-insensitive from pH 4 to 10 and minimal quenching when conjugated to proteins
  • High Photostability: Ensures reliable performance for multicolor flow cytometry and advanced imaging applications like STORM

Product description

XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor™ 405 (ThermoFisher). This dye is water-soluble and optimized for excitation by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability.

XFD405 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.300.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.430.56
XFD532 amine534553810000.240.09
XFD555 amine5535681550000.080.08
XFD568 amine579603913000.450.46
XFD647 amine650671270000-0.03
XFD750 amine752776290000-0.04
XFD350 amine343441190000.250.19
XFD405 Tetrazine40142135,0000.230.70
XFD405 TCO40142135,0000.230.70
XFD430 amine43254015,000-0.28
XFD514 amine518543800000.310.18
XFD546 amine5615721120000.210.12
XFD610 amine611629144,0000.430.44
ICG amine789813230000-0.076
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Page updated on December 6, 2024

Ordering information

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Catalog Number70012
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

671.71

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.23

Correction Factor (280 nm)

0.70

Extinction coefficient (cm -1 M -1)

35,000

Excitation (nm)

401

Emission (nm)

421

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200