XFD405 amine
Product key features
- Ex/Em: 401/421 nm
- Extinction coefficient: 35,000 cm-1M-1
- Reactive Group: Amine
- Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
- Consistent Fluorescence: pH-insensitive from pH 4 to 10 and minimal quenching when conjugated to proteins
- High Photostability: Ensures reliable performance for multicolor flow cytometry and advanced imaging applications like STORM
Product description
XFD405, manufactured by AAT Bioquest, is a blue-fluorescent dye that is structurally identical to Alexa Fluor™ 405 (ThermoFisher). This dye is water-soluble and optimized for excitation by the 407 nm krypton laser line or the 408 nm violet laser diode, making it suitable for a range of fluorescence-based techniques. XFD405 is pH-insensitive across a wide range (pH 4 - 10) and exhibits minimal quenching when conjugated to proteins, ensuring consistent fluorescence signals in live-cell imaging. With an excitation maximum at 401 nm and emission at 422 nm, XFD405 is well-suited for multicolor flow cytometry and super-resolution microscopy (STORM), providing reliable performance in applications requiring distinct spectral separation and photostability.
XFD405 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.30 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.43 | 0.56 |
XFD532 amine | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD555 amine | 553 | 568 | 155000 | 0.08 | 0.08 |
XFD568 amine | 579 | 603 | 91300 | 0.45 | 0.46 |
XFD647 amine | 650 | 671 | 270000 | - | 0.03 |
XFD750 amine | 752 | 776 | 290000 | - | 0.04 |
XFD350 amine | 343 | 441 | 19000 | 0.25 | 0.19 |
XFD405 Tetrazine | 401 | 421 | 35,000 | 0.23 | 0.70 |
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