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AAT Bioquest

XFD430 amine

Product key features

  • Ex/Em: 432/540 nm
  • Extinction coefficient: 15,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • pH-Insensitive & High Hydrophilicity: Maintains stable fluorescence across pH 4–10 and minimizes aggregation
  • Large Stokes Shift: Provides clear signal separation and minimizes spectral overlap, enhancing imaging accuracy in sensitive applications

Product description

XFD430, manufactured by AAT Bioquest, is a green-fluorescent dye that is structurally identical to Alexa Fluor™ 430 (ThermoFisher). It has excitation and emission maxima of 432 nm and 540 nm, respectively, and is efficiently excited by a 405 nm violet laser. Its large Stokes shift minimizes spectral overlap with blue-emitting fluorophores, significantly improving the signal-to-noise ratio in multiplexed fluorescence assays. XFD430 also exhibits excellent water solubility and remains pH-insensitive across a wide range (pH 4–10), making it well-suited for generating stable, reproducible fluorescence signals in both microscopy and flow cytometry applications.

XFD430 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Page updated on April 15, 2025

Ordering information

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Catalog Number70023
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

545.57

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.28

Extinction coefficient (cm -1 M -1)

15,000

Excitation (nm)

432

Emission (nm)

540

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
Product Image
Gallery Image 1
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.