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AAT Bioquest

XFD430 amine

Product key features

  • Ex/Em: 432/540 nm
  • Extinction coefficient: 15,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • pH-Insensitive & High Hydrophilicity: Maintains stable fluorescence across pH 4–10 and minimizes aggregation
  • Large Stokes Shift: Provides clear signal separation and minimizes spectral overlap, enhancing imaging accuracy in sensitive applications

Product description

XFD430, manufactured by AAT Bioquest, is a green-fluorescent dye that is structurally identical to Alexa Fluor™ 430 (ThermoFisher). It has excitation and emission maxima of 432 nm and 540 nm, respectively, and is efficiently excited by a 405 nm violet laser. Its large Stokes shift minimizes spectral overlap with blue-emitting fluorophores, significantly improving the signal-to-noise ratio in multiplexed fluorescence assays. XFD430 also exhibits excellent water solubility and remains pH-insensitive across a wide range (pH 4–10), making it well-suited for generating stable, reproducible fluorescence signals in both microscopy and flow cytometry applications.

XFD430 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.56
XFD532 amine534553810000.09
XFD555 amine5535681550000.08
XFD568 amine579603913000.46
XFD647 amine6506712700000.03
XFD750 amine7527762900000.04
XFD350 amine343441190000.19
XFD405 amine40142135,0000.70
XFD430 TCO43254015,0000.28
XFD430 Tetrazine43254015,0000.28
XFD514 amine518543800000.18
XFD546 amine5615721120000.12
XFD610 amine611629144,0000.44
ICG amine7898132300000.076
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Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number70023
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

545.57

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.28

Extinction coefficient (cm -1 M -1)

15,000

Excitation (nm)

432

Emission (nm)

540

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200