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AAT Bioquest

XFD430 Tetrazine

Product key features

  • Ex/Em: 432/540 nm
  • Extinction coefficient: 15,000 cm-1M-1
  • Reactive Group: Tetrazine
  • Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
  • pH-Insensitive & High Hydrophilicity: Maintains stable fluorescence across pH 4–10 and minimizes aggregation
  • Large Stokes Shift: Provides clear signal separation and minimizes spectral overlap, enhancing imaging accuracy in sensitive applications

Product description

XFD430, manufactured by AAT Bioquest, is a green-fluorescent dye that is structurally identical to Alexa Fluor™ 430 (ThermoFisher). With excitation and emission maxima at 432 nm and 540 nm, respectively, it is optimally excited by a 405 nm violet laser. Its large Stokes shift reduces spectral overlap with blue-emitting fluorophores, providing a superior signal-to-noise ratio in multiplexed fluorescence assays. XFD430 also exhibits good water solubility and remains pH-insensitive across a wide range (pH 4–10), making it well-suited for generating stable, reproducible fluorescence signals in both microscopy and flow cytometry applications.

XFD430 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Page updated on April 25, 2025

Ordering information

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Catalog Number70028
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

787.90

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.28

Extinction coefficient (cm -1 M -1)

15,000

Excitation (nm)

432

Emission (nm)

540

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Product Image
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Gallery Image 1
Schematic illustrating the strain‐promoted inverse‐electron‐demand Diels–Alder (iEDDA) reaction between a tetrazine–dye conjugate and a trans‐cyclooctene (TCO)–modified biomolecule. The tetrazine–dye selectively and rapidly reacts with the TCO moiety on the biomolecule to form a stable linkage, labeling the nucleic acid with the dye. This bioorthogonal “click chemistry” process is fast, selective, and well‐suited for biocompatible labeling applications.