Name: XFD488 aldehyde
Brand: AAT Bioquest
SKU: 9015
Availability: InStock

XFD488 aldehyde

XFD488 Aldehyde is a amine, hydrazine, or hydroxylamine-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Versatile Conjugation: Reacts with amines, hydrazides, or hydroxylamines from pH 5–9, and can target N-terminal amine groups at acidic pH
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4-10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Chemical structure for XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*.

Datasheet

Catalog	Size	Price	Quantity
9015	5 mg	Price
9016	1 mg	Price

Overview

XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). XFD488 aldehyde contains the fluorophore of XFD488. It is a reactive green fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5–9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated XFD488 dye can be easily detected by a common fluorescence instrument under FITC channel.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 aldehyde to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.2 µL	666.001 µL	1.332 mL	6.66 mL	13.32 mL
5 mM	26.64 µL	133.2 µL	266.4 µL	1.332 mL	2.664 mL
10 mM	13.32 µL	66.6 µL	133.2 µL	666.001 µL	1.332 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	750.75 g/mol	=	mL	x	mM	=	mmol

References

View all 10 references: Citation Explorer

Optimization of 3-aminopropyltriethoxysilane functionalization on silicon nitride surface for biomolecule immobilization.

Authors:

Saengdee, Pawasuth and Promptmas, Chamras and Thanapitak, Surachoke and Srisuwan, Awirut and Pankiew, Apirak and Thornyanadacha, Nutthaphat and Chaisriratanakul, Woraphan and Chaowicharat, Ekalak and Jeamsaksiri, Wutthinan

Journal:

Talanta (2020): 120305

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments.

Authors:

Grate, Jay W and Mo, Kai-For and Shin, Yongsoon and Vasdekis, Andreas and Warner, Marvin G and Kelly, Ryan T and Orr, Galya and Hu, Dehong and Dehoff, Karl J and Brockman, Fred J and Wilkins, Michael J

Journal:

Bioconjugate chemistry (2015): 593-601

Simultaneous visualization and cell-specific confirmation of RNA and protein in the mouse retina.

Authors:

Stempel, Andrew J and Morgans, Catherine W and Stout, J Timothy and Appukuttan, Binoy

Journal:

Molecular vision (2014): 1366-73

Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.

Authors:

Dirksen, Anouk and Dawson, Philip E

Journal:

Bioconjugate chemistry (2008): 2543-8

Raman nanoparticle probes for antibody-based protein detection in tissues.

Authors:

Lutz, Barry and Dentinger, Claire and Sun, Lei and Nguyen, Lienchi and Zhang, Jingwu and Chmura, Aj and Allen, April and Chan, Selena and Knudsen, Beatrice

Journal:

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2008): 371-9

Physical properties

Molecular weight	750.75
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on January 15, 2026