Calculator
XFD488 alkyne
Product key features
- Ex/Em: 499/520 nm
- Extinction coefficient: 71,000 cm-1M-1
- Reactive Group: Alkyne
- Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
- Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
- Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
The fluorescent dye alkynes react with azides via a copper-catalyzed click reaction (CuAAC). This click chemistry is increasingly being used in a variety of biological applications. XFD488 alkyne has a chemical structure similar to Alexa Fluor® 488 alkyne (Alexa Fluor® is the Trademark of Thermo Fisher Scientific). XFD488 alkyne is reactive with terminal alkynes via a copper-catalyzed click reaction as a bio-orthogonal or biologically unique hapten for use in applications requiring signal amplification.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 129.212 µL | 646.062 µL | 1.292 mL | 6.461 mL | 12.921 mL |
| 5 mM | 25.842 µL | 129.212 µL | 258.425 µL | 1.292 mL | 2.584 mL |
| 10 mM | 12.921 µL | 64.606 µL | 129.212 µL | 646.062 µL | 1.292 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD350 alkyne | 343 | 441 | 19000 | 0.25 | 0.19 |
| XFD405 alkyne | 401 | 421 | 35,000 | 0.23 | 0.70 |
| XFD430 alkyne | 432 | 540 | 15,000 | - | 0.28 |
| XFD488 aldehyde | 499 | 520 | 71000 | 0.30 | 0.11 |
| XFD488 Hydroxylamine | 499 | 520 | 71000 | 0.30 | 0.11 |
| XFD488 TCO | 499 | 520 | 73000 | 0.3 | 0.11 |
| XFD488 tetrazine | 499 | 520 | 71000 | 0.30 | 0.11 |
| XFD514 alkyne | 518 | 543 | 80000 | 0.31 | 0.18 |
| XFD532 alkyne | 534 | 553 | 81000 | 0.24 | 0.09 |
Show More (16) | |||||
References
View all 39 references: Citation Explorer
Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Authors: Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.
Journal: Anal Biochem (2017): 13-22
Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Authors: Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.
Journal: Methods Mol Biol (2017): 21-Sep
Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Authors: Maroteaux, M.; Liu, S. J.
Journal: eNeuro (2016)
Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
Authors: Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.
Journal: Bioconjug Chem (2015): 593-601
In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Authors: Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.
Journal: Neurosurg Focus (2014): E12
Page updated on August 11, 2025
Certificate of origin