XFD488 alkyne

XFD488 alkyne is an azide-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an alkyne‐functionalized dye and an azide‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions, tolerates a wide range of functional groups, and is frequently used to label proteins, nucleic acids, and other biomolecules for imaging, proteomics, and high‐throughput assays.

Catalog	Size	Price	Quantity
1700	1 mg	Price

Overview

The fluorescent dye alkynes react with azides via a copper-catalyzed click reaction (CuAAC). This click chemistry is increasingly being used in a variety of biological applications. XFD488 alkyne has a chemical structure similar to Alexa Fluor® 488 alkyne (Alexa Fluor® is the Trademark of Thermo Fisher Scientific). XFD488 alkyne is reactive with terminal alkynes via a copper-catalyzed click reaction as a bio-orthogonal or biologically unique hapten for use in applications requiring signal amplification.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	129.212 µL	646.062 µL	1.292 mL	6.461 mL	12.921 mL
5 mM	25.842 µL	129.212 µL	258.425 µL	1.292 mL	2.584 mL
10 mM	12.921 µL	64.606 µL	129.212 µL	646.062 µL	1.292 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	773.92 g/mol	=	mL	x	mM	=	mmol

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

Authors:

Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.

Journal:

Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells

Authors:

Maroteaux, M.; Liu, S. J.

Journal:

eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments

Authors:

Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.

Journal:

Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies

Authors:

Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.

Journal:

Neurosurg Focus (2014): E12

Physical properties

Molecular weight	773.92
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 20, 2025