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XFD488 amine
XFD488 amine is a carbonyl-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
protocol
Protocol
sds
SDS
COO
COA
CatalogSize
Price
Quantity
17051 mg
Price
 
Physical properties
Molecular weight719.83
SolventDMSO
Spectral properties
Correction factor (260 nm)0.30
Correction factor (280 nm)0.11
Extinction coefficient (cm -1 M -1)
71000
Excitation (nm)499
Emission (nm)520
Quantum yield
0.92
1
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on October 13, 2025