Name: XFD488 C5 Maleimide
Brand: AAT Bioquest
SKU: 1878
Price: 232 USD
Availability: InStock

XFD488 C5 Maleimide

XFD488 C5 maleimide is a thiol-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Versatile Conjugation: Efficiently labels thiol groups on proteins, antibodies, and oligonucleotides with stable thioether bonds
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Schematic illustrating the maleimide–thiol reaction between a maleimide‐functionalized dye and a thiol‐modified biomolecule. This method is widely used for site‐specific conjugation to cysteine residues in proteins or to thiolated oligonucleotides. Maleimide–thiol coupling proceeds efficiently at or near physiological pH and results in a stable thioether bond.

Catalog	Size	Price	Quantity
1878	1 mg	Price

Citations

View all 3 citations: Citation Explorer

Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions

Authors:

Lefebvre, Sol{\`e}ne N and Nijland, Mark and Maslov, Ivan and Slotboom, Dirk J

Journal:

Nature Communications (2025): 1--12

Bidirectional ATP-driven transport of cobalamin by the mycobacterial ABC transporter BacA

Authors:

Nijland, Mark and Lefebvre, Sol{\`e}ne N and Thangaratnarajah, Chancievan and Slotboom, Dirk J

Journal:

Nature Communications (2024): 2626

Kinetic quantitative analysis reveals the suppression of Sup35NM amyloid fibril nucleation by liquid-liquid phase separation.

Authors:

Fukuyama, Mao and Nishinami, Suguru and Maruyama, Yoko and Ozawa, Taiki and Tomita, Shunsuke and Ohhashi, Yumiko and Kasuya, Motohiro and Gen, Masao and Chatani, Eri and Shiraki, Kentaro and others,

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

Authors:

Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.

Journal:

Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells

Authors:

Maroteaux, M.; Liu, S. J.

Journal:

eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments

Authors:

Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.

Journal:

Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies

Authors:

Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.

Journal:

Neurosurg Focus (2014): E12

Physical properties

Molecular weight	698.67
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025