Name: XFD488 NHS Ester
Brand: AAT Bioquest
SKU: 1812
Availability: InStock

XFD488 NHS Ester

XFD488 NHS Ester is an amine-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Easy Conjugation: Efficiently labels primary amines on proteins, ligands, and amine-modified oligonucleotides
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Schematic illustrating the NHS ester–amine coupling between an NHS ester‐activated dye and an amine‐modified biomolecule. NHS esters react readily with lysine side‐chain amines or N‐terminal amines on proteins, peptides, and amine‐modified oligonucleotides under mild conditions (pH 7–9). The resulting amide bond is stable and physiologically compatible.

Protocol

COO

COA

Catalog	Size	Price	Quantity
1812	1 mg	Price
71902	5 mg	Price

Citations

View all 12 citations: Citation Explorer

$\beta$-propeller protein-associated neurodegeneration protein WDR45 regulates stress granule disassembly via phase separation with Caprin-1

Authors:

Li, Yin and Fang, Jie and Ding, Yuqi and Zhang, Xilong and Liu, Ying and Qiu, Wanting and Xu, He and Kang, Yunzhe and Chen, Jiayu and Gao, Yanyan and others,

Journal:

Nature Communications (2025): 1--18

Effectiveness evaluation of fluorescent compensation in multicolor flow cytometry: A quantitative study

Authors:

Fan, Long and Gao, Chiyuan and Wang, Junbo and Huo, Xiaoye and Chen, Jian

Journal:

Nanotechnology and Precision Engineering (2025)

Exploring the anticancer potential of Cytotoxin 10 from Naja kaouthia venom: Mechanistic insights from breast and lung cancer cell lines

Authors:

Basumatary, Mandira and Talukdar, Amit and Sharma, Manoj and Dutta, Anupam and Mukhopadhyay, Rupak and Doley, Robin

Journal:

Chemico-Biological Interactions (2024): 111254

Concomitant silanization and controlled fibronectin adsorption on S53P4 bioactive glass enhances human adipose stem cells spreading and differentiation

Authors:

Gobbo, Virginia Alessandra and Houaoui, Amel and Tajik, Kimiya and Hyt{\"o}nen, Vesa P and Miettinen, Susanna and Massera, Jonathan

Journal:

Applied Surface Science Advances (2024): 100635

The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network

Authors:

Yao, Zhiying and Liu, Yi and Chen, Qi and Chen, Xiaoxin and Zhu, Zhenshuo and Song, Sha and Ma, Xianjue and Yang, Peiguo

Journal:

Cell Reports (2024)

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

Authors:

Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.

Journal:

Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells

Authors:

Maroteaux, M.; Liu, S. J.

Journal:

eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments

Authors:

Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.

Journal:

Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies

Authors:

Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.

Journal:

Neurosurg Focus (2014): E12

Physical properties

Molecular weight	833.93
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 23, 2025