Name: XFD488 NHS Ester-UltraPure Grade
Brand: AAT Bioquest
SKU: 1810
Availability: InStock

XFD488 NHS Ester-UltraPure Grade

XFD488 NHS Ester Ultrapure Grade is an amine-reactive, green fluorescent dye with an excitation/emission of 499/520 nm that is used for flow cytometry and microscopy applications.

Easy Conjugation: Efficiently labels primary amines on proteins, ligands, and amine-modified oligonucleotides
Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
>75% Purity: Exceeds industry standards and eliminates non-fluorescent XFD488 Bis-NHS ester impurity

Schematic illustrating the NHS ester–amine coupling between an NHS ester‐activated dye and an amine‐modified biomolecule. NHS esters react readily with lysine side‐chain amines or N‐terminal amines on proteins, peptides, and amine‐modified oligonucleotides under mild conditions (pH 7–9). The resulting amide bond is stable and physiologically compatible.

Protocol

COO

COA

Catalog	Size	Price	Quantity
1810	1 mg	Price
71901	5 mg	Price

Overview

XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). XFD488 NHS Ester (Succinimidyl Ester) has the same molecule as Alexa Fluor® 488 NHS Ester (#A20000 and A20100 from Thermo Fisher). It is a bright green-fluorescent dye optimal for use with the 488 nm Argon laser. XFD488 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD488 NHS ester (or succinimidyl ester) is the most convenient amine-reactive form for conjugating this dye to a protein or antibody. Although the Alexa Fluor® 488 NHS Ester is one of the most common green fluorescent labeling dyes, its purity has been quite challenging. Thermo Fisher provides it with purity of ~50–60% while a few other vendors offer it at even lower purities. Among all the impurities, we noted that the Bis-NHS ester of XFD488 is quite detrimental since it could give a non-fluorescent product. This high purity XFD488 NHS ester is free of the detrimental XFD488 Bis-NHS ester impurity with the minimal purity of >75%.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 NHS Ester-UltraPure Grade to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	155.424 µL	777.122 µL	1.554 mL	7.771 mL	15.542 mL
5 mM	31.085 µL	155.424 µL	310.849 µL	1.554 mL	3.108 mL
10 mM	15.542 µL	77.712 µL	155.424 µL	777.122 µL	1.554 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	643.4 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

In vitro cytocompatibility of antibacterial silver and copper-doped bioactive glasses

Authors:

Lallukka, Mari and Houaoui, Amel and Miola, Marta and Miettinen, Susanna and Massera, Jonathan and Vern{\'e}, Enrica

Journal:

Ceramics International (2023)

An ULK1/2-PXN mechanotransduction complex suppresses breast cancer cell migration

Authors:

Liang, Peigang and Zhang, Jiaqi and Wu, Yuchen and Zheng, Shanyuan and Xu, Zhaopeng and Yang, Shuo and Wang, Jinfang and Ma, Suibin and Xiao, Li and Wu, Tianhui and others,

Journal:

bioRxiv (2023): 2023--02

Physical properties

Molecular weight	643.40
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025