XFD488 TCO

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	856.79
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

iFluor® 488 TCO

Overview SDS Protocol

Upgrade: iFluor® 488 TCO

Molecular weight

856.79

Correction Factor (260 nm)

0.3

Correction Factor (280 nm)

0.11

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

499

Emission (nm)

520

Quantum yield

0.92¹

XFD488 contains the same fluorophore of Alexa Fluor® 488. It is a bright, green-fluorescent dye with excitation ideally suited for the 488 nm laser line. It is used for stable signal generation in imaging and flow cytometry. AF488 TCO demonstrates exceptionally fast reaction with tetrazines, the fastest kinetics ever reported for any biorthogonal reaction. It provides a convenient tool for making biological conjugates with tetrazine-modified substrates. The resulted conjugates provide the bright, green fluorescence of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher Scientific).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	116.715 µL	583.574 µL	1.167 mL	5.836 mL	11.671 mL
5 mM	23.343 µL	116.715 µL	233.429 µL	1.167 mL	2.334 mL
10 mM	11.671 µL	58.357 µL	116.715 µL	583.574 µL	1.167 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	490	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	490	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	490	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	490	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	490	520	71000	0.92¹	0.30	0.11
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	490	520	71000	0.92¹	0.30	0.11
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	490	520	71000	0.92¹	0.30	0.11
Cy3B TCO	560	571	120000¹	0.58¹	0.048	0.069

Images

Figure 1. Chemical structure for XFD488 TCO.

References

View all 50 references: Citation Explorer

Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449

A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414

Introducing Cysteines into Nanobodies for Site-Specific Labeling.
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343

Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6

Gold nanoparticles enhance fluorescence signals by flow cytometry at low antibody concentrations.
Authors: Reis, Daniela S and de Oliveira, Vivian L and Silva, Misael L and Paniago, Roberto M and Ladeira, Luiz O and Andrade, Lidia M
Journal: Journal of materials chemistry. B (2021): 1414-1423

Binding of cytochrome P450 27C1, a retinoid desaturase, to its accessory protein adrenodoxin.
Authors: Glass, Sarah M and Webb, Stephany N and Guengerich, F Peter
Journal: Archives of biochemistry and biophysics (2021): 109076

Pegylated-Catalase Is Protective in Lung Ischemic Injury and Oxidative Stress.
Authors: Kim, Jung-Lye and Reader, Brenda F and Dumond, Curtis and Lee, Yonggyu and Mokadam, Nahush A and Black, Sylvester M and Whitson, Bryan A
Journal: The Annals of thoracic surgery (2021): 1019-1027

Retinal ganglion cells projecting to superior colliculus and pulvinar in marmoset.
Authors: Grünert, Ulrike and Lee, Sammy C S and Kwan, William C and Mundinano, Inaki-Carril and Bourne, James A and Martin, Paul R
Journal: Brain structure & function (2021)

An In Vitro Assay to Monitor Sertoli Cell Blood-Testis Barrier (BTB) Integrity.
Authors: Wu, Siwen and Wang, Lingling and Tang, Elizabeth I and Wang, Junlu and Cheng, C Yan
Journal: Methods in molecular biology (Clifton, N.J.) (2021)

A fully integrated isotachophoresis with a programmable microfluidic platform.
Authors: Shebindu, Adam and Somaweera, Himali and Estlack, Zachary and Kim, Jungtae and Kim, Jungkyu
Journal: Talanta (2021): 122039