Name: XFD488-Wheat Germ Agglutinin (WGA) Conjugate
Brand: AAT Bioquest
SKU: 25500
Price: 111 USD
Availability: InStock

XFD488-Wheat Germ Agglutinin (WGA) Conjugate

XFD488 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 488 (Alexa Fluor® is the trademark of Thermo Fisher). Wheat germ agglutinin (WGA) is a lectin that binds to N-acetyl-D-glucosamine and sialic acid. It is of the most studied and useful lectins for its biological applications. Since WGA binds to glycoconjugates its derivatives and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. The carbohydrate-binding specificity of WGA is directed against sequences of β-1,4-GlcNAc-linked residues, the chitodextrins. Each monomer contains two identical, non-interacting binding sites, which are complementary to 3 or 4 β-1,4-GlcNAc units. Of the monosaccharides examined, only GlcNAc binds to WGA. ManNAc does not bind, and GalNAc binds only weakly. XFD488 conjugate of WGA is equivalent to the Alexa Fluor® 488 conjugate of WGA. It exhibits bright, green fluorescence. XFD488 WGA conjugate binds to sialic acid and N-acetylglucosaminyl residues.

Live HeLa cells were stained with Wheat Germ Agglutinin, XFD488 labeled at 5 µg/mL for 30 minutes followed by Hoechst 33342 (Cat No. 17535). Image was acquired using fluorescence microscopy using FITC and DAPI filter set.

Catalog	Size	Price	Quantity
25500	1 mg	Price

References

View all 50 references: Citation Explorer

Wheat germ agglutinin is involved in the protective action of 24-epibrassinolide on the roots of wheat seedlings under drought conditions.

Authors:

Avalbaev, Azamat and Bezrukova, Marina and Allagulova, Chulpan and Lubyanova, Alsu and Kudoyarova, Guzel and Fedorova, Kristina and Maslennikova, Dilara and Yuldashev, Ruslan and Shakirova, Farida

Journal:

Plant physiology and biochemistry : PPB (2020): 420-427

Membrane-associated gamma-glutamyl transferase and alkaline phosphatase in the context of concanavalin A- and wheat germ agglutinin-reactive glycans mark seminal prostasome populations from normozoospermic and oligozoospermic men.

Authors:

Janković, Tamara and Goč, Sanja and Mitić, Ninoslav and Danilović Luković, Jelena and Janković, Miroslava

Journal:

Upsala journal of medical sciences (2020): 10-18

Succinylated Wheat Germ Agglutinin Colocalizes with the Toxoplasma gondii Cyst Wall Glycoprotein CST1.

Authors:

Guevara, Rebekah B and Fox, Barbara A and Bzik, David J

Journal:

mSphere (2020)

Wheat germ agglutinin is a biomarker of whole grain content in wheat flour and pasta.

Authors:

Killilea, David W and McQueen, Rebecca and Abegania, Judi R

Journal:

Journal of food science (2020): 808-815

Wheat germ agglutinin liposomes with surface grafted cyclodextrins as bioadhesive dual-drug delivery nanocarriers to treat oral cells.

Authors:

Wijetunge, Sashini S and Wen, Jianchuan and Yeh, Chih-Ko and Sun, Yuyu

Journal:

Colloids and surfaces. B, Biointerfaces (2020): 110572

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.30
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	71000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on April 24, 2026