XFD514 acid
| Overview |  SDSProtocol |
Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 518 | Emission (nm) 543 |
XFD514 is manufactured by AAT Bioquest, and it has the same chemical structure to Alexa Fluor® 514 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright yellow-fluorescent dye that can be used to replace rhodamine 6G with dramatically improved water solubility. XFD514 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD514 acid is the most economic amine-reactive form for conjugating Alexa Fluor® 514 to a peptide.
Spectrum
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Spectral properties
| Extinction coefficient (cm -1 M -1) | 80000 |
| Excitation (nm) | 518 |
| Emission (nm) | 543 |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | - |
| XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 73000 | - |
| XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | - |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | - |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 88000 | - |
| XFD514 tyramide | 518 | 543 | 80000 | 0.18 |
| ICG acid | 789 | 814 | - | 0.076 |
| Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.073 |
| Cy5NS acid | 651 | 670 | 2500001 | 0.03 |
Show More (18) | ||||
Images
Figure 1. Chemical structure for XFD 514 acid.
References
View all 2 references: Citation Explorer
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41