XFD514 acid
XFD514 is manufactured by AAT Bioquest, and it has the same chemical structure to Alexa Fluor® 514 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright yellow-fluorescent dye that can be used to replace rhodamine 6G with dramatically improved water solubility. XFD514 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD514 acid is the most economic amine-reactive form for conjugating Alexa Fluor® 514 to a peptide.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
XFD514 tyramide | 518 | 543 | 80000 | - | 0.31 | 0.18 |
XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
XFD647 acid | 650 | 671 | 270000 | 0.331 | - | 0.03 |
XFD750 acid | 752 | 776 | 290000 | 0.121 | - | 0.04 |
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References
View all 2 references: Citation Explorer
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41