XFD514 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 730.64 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.18 |
| Extinction coefficient (cm -1 M -1) | 80000 |
| Excitation (nm) | 315 |
| Emission (nm) | 543 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
Molecular weight 730.64 | Correction Factor (260 nm) 0.31 | Correction Factor (280 nm) 0.18 | Extinction coefficient (cm -1 M -1) 80000 | Excitation (nm) 315 | Emission (nm) 543 |
XFD514 is manufactured by AAT Bioquest, and it has the same chemical structure to Alexa Fluor® 514 (Alexa Fluor® is the trademark of ThermoFisher). It is a bright yellow-fluorescent dye that can be used to replace rhodamine 6G with dramatically improved water solubility. XFD514 dye is water soluble and pH-insensitive from pH 4 to pH 10. XFD514 acid is the most economic amine-reactive form for conjugating Alexa Fluor® 514 to a peptide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD514 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 136.866 µL | 684.332 µL | 1.369 mL | 6.843 mL | 13.687 mL |
| 5 mM | 27.373 µL | 136.866 µL | 273.733 µL | 1.369 mL | 2.737 mL |
| 10 mM | 13.687 µL | 68.433 µL | 136.866 µL | 684.332 µL | 1.369 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.31 |
| Correction Factor (280 nm) | 0.18 |
| Extinction coefficient (cm -1 M -1) | 80000 |
| Excitation (nm) | 315 |
| Emission (nm) | 543 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 317 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 303 | 439 | 19000 | - | 0.25 | 0.19 |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 323 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 546 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
| XFD514 tyramide | 518 | 543 | 80000 | - | 0.31 | 0.18 |
| XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD647 acid | 650 | 671 | 270000 | 0.331 | - | 0.03 |
| XFD750 acid | 752 | 776 | 290000 | 0.121 | - | 0.04 |
Show More (12) | ||||||
Images
Figure 1. Chemical structure for XFD 514 acid.
References
View all 2 references: Citation Explorer
Toxicity of organic fluorophores used in molecular imaging: literature review.
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Authors: Alford, Raphael and Simpson, Haley M and Duberman, Josh and Hill, G Craig and Ogawa, Mikako and Regino, Celeste and Kobayashi, Hisataka and Choyke, Peter L
Journal: Molecular imaging (2009): 341-54
Single-molecule dynamics of phytochrome-bound fluorophores probed by fluorescence correlation spectroscopy.
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41
Authors: Miller, Abigail E and Fischer, Amanda J and Laurence, Ted and Hollars, Christopher W and Saykally, Richard J and Lagarias, J Clark and Huser, Thomas
Journal: Proceedings of the National Academy of Sciences of the United States of America (2006): 11136-41