XFD514 amine
Product key features
- Ex/Em: 518/543 nm
- Extinction coefficient: 80,000 cm-1M-1
- Reactive Group: Amine
- Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
- Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
- Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays.
XFD514 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.30 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.43 | 0.56 |
XFD514 tyramide | 518 | 543 | 80000 | 0.31 | 0.18 |
XFD532 amine | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD555 amine | 553 | 568 | 155000 | 0.08 | 0.08 |
XFD568 amine | 579 | 603 | 91300 | 0.45 | 0.46 |
XFD647 amine | 650 | 671 | 270000 | - | 0.03 |
XFD750 amine | 752 | 776 | 290000 | - | 0.04 |
XFD350 amine | 343 | 441 | 19000 | 0.25 | 0.19 |
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