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AAT Bioquest

XFD514 amine

Product key features

  • Ex/Em: 518/543 nm
  • Extinction coefficient: 80,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays.

XFD514 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.300.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.430.56
XFD514 tyramide518543800000.310.18
XFD532 amine534553810000.240.09
XFD555 amine5535681550000.080.08
XFD568 amine579603913000.450.46
XFD647 amine650671270000-0.03
XFD750 amine752776290000-0.04
XFD350 amine343441190000.250.19
XFD405 amine40142135,0000.230.70
XFD430 amine43254015,000-0.28
XFD514 TCO518543800000.310.18
XFD514 Tetrazine518543800000.310.18
XFD546 amine5615721120000.210.12
XFD610 amine611629144,0000.430.44
ICG amine789813230000-0.076
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Page updated on December 6, 2024

Ordering information

Price
Unit size
Catalog Number70043
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

772.72

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.31

Correction Factor (280 nm)

0.18

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

518

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501