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AAT Bioquest

XFD514 amine

Product key features

  • Ex/Em: 518/543 nm
  • Extinction coefficient: 80,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications

Product description

XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays.

XFD514 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Page updated on April 22, 2025

Ordering information

Price
Unit size
Catalog Number70043
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Physical properties

Molecular weight

772.72

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.31

Correction Factor (280 nm)

0.18

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

518

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.