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AAT Bioquest

XFD514 PEG4 DBCO

Product key features

  • Ex/Em: 518/543 nm
  • Extinction coefficient: 80,000 cm-1M-1
  • Reactive Group: DBCO
  • Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via copper-free SPAAC
  • Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
  • Advanced PEG4 Spacer: Enhances solubility and minimizes steric hindrance for improved biocompatibility

Product description

XFD514, manufactured by AAT Bioquest, is a bright green-fluorescent dye that is structurally identical to Alexa Fluor™ 514 (ThermoFisher). With optimal excitation between 480-535 nm, it is ideally compatible with the 488 nm line of the Argon-Ion laser and the 532 nm line of the frequency-doubled Nd:YAG laser. XFD514 demonstrates enhanced water solubility due to the incorporation of a PEG4 linker and remains stable over a wide pH range (pH 4–10), providing consistent and robust fluorescence signals. These properties make it highly suitable for advanced imaging techniques such as fluorescence microscopy and flow cytometry. XFD514 is a suitable replacement for rhodamine 6G.

The DBCO derivative of XFD514 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD514 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 PEG4 DBCO499520710000.9210.300.11
XFD350 PEG4 DBCO34344119000-0.250.19
XFD405 PEG4 DBCO40142135,000-0.230.70
XFD430 PEG4 DBCO43254015,000--0.28
XFD546 PEG4 DBCO5615721120000.7910.210.12
XFD532 PEG4 DBCO534553810000.6110.240.09
XFD568 PEG4 DBCO579603913000.6910.450.46
XFD594 PEG4 DBCO590618900000.6610.430.56
Page updated on November 7, 2024

Ordering information

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Catalog Number70046
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

1324.61

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.31

Correction Factor (280 nm)

0.18

Extinction coefficient (cm -1 M -1)

80000

Excitation (nm)

518

Emission (nm)

543

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501