XFD514 TCO
Product key features
- Ex/Em: 518/543 nm
- Extinction coefficient: 80,000 cm-1M-1
- Reactive Group: TCO
- Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
- Bright and Stable: Offers high fluorescence intensity, photostability, and consistent performance across pH 4—10
- Hydrophilic: Prevents aggregation and enhances signal clarity for advanced imaging and live-cell applications
Product description
XFD514, manufactured by AAT Bioquest, is a green-fluorescent dye structurally identical to Alexa Fluor™ 514 (ThermoFisher). It is characterized by high photostability and exceptional brightness, making it highly suitable for advanced imaging and flow cytometry applications. The dye is water-soluble and remains pH-insensitive within a broad range (pH 4–10), ensuring consistent fluorescence performance across diverse experimental conditions. XFD514 offers robust and reliable labeling, enabling precise and reproducible results in fluorescence-based assays. It also serves as an effective replacement for rhodamine 6G in applications requiring enhanced performance.
XFD514 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.