Name: XFD532 tyramide
Brand: AAT Bioquest
SKU: 11072
Price: 213 USD
Availability: InStock

XFD532 tyramide

XFD532 Tyramide is a yellow fluorescent dye with an excitation/emission of 534/553 nm that is used for flow cytometry and microscopy applications.

Tyramide Signal Amplification (TSA): Facilitates ultrasensitive detection of low-abundance targets, ideal for IHC and ICC applications
Bright & Stable XFD532 Dye: Delivers high quantum yield with robust resistance to photobleaching and pH variations (4–10)

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong> ™</strong> amplified methods. Human lung adenocarcinoma positive tissue sections were stained with Mouse anti-EpCAM or Control Mouse IgG antibody and then incubated with polyHRP-labeled Goat anti-Mouse IgG secondary antibody followed by XFD 532 tyramide (Cat#11072).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11072	200 Slides	Price

Overview

Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD 532 tyramide contains the Alexa Fluor® 532 fluorophore that can be readily detected with the Alexa Fluor® 532/ATTO 532 filter set (Alexa Fluor® is the trademark of ThermoFisher). XFD 532 tyramide has intense yellow-orange fluorescence color.

References

View all 13 references: Citation Explorer

Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Carboxylcytosine in Sections of Zebrafish Embryos.

Authors:

Jessop, Peter and Gering, Martin

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 193-208

Ultrastructure of light-activated axons following optogenetic stimulation to produce late-phase long-term potentiation.

Authors:

Kuwajima, Masaaki and Ostrovskaya, Olga I and Cao, Guan and Weisberg, Seth A and Harris, Kristen M and Zemelman, Boris V

Journal:

PloS one (2020): e0226797

NIR-labeled perfluoropolyether nanoemulsions for drug delivery and imaging.

Authors:

O'Hanlon, Claire E and Amede, Konjit G and O'Hear, Meredith R and Janjic, Jelena M

Journal:

Journal of fluorine chemistry (2012): 27-33

Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry.

Authors:

Clutter, Matthew R and Heffner, Garrett C and Krutzik, Peter O and Sachen, Kacey L and Nolan, Garry P

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2010): 1020-31

Methoxychlor and estradiol induce oxidative stress DNA damage in the mouse ovarian surface epithelium.

Authors:

Symonds, Daniel A and Merchenthaler, Istvan and Flaws, Jodi A

Journal:

Toxicological sciences : an official journal of the Society of Toxicology (2008): 182-7

Physical properties

Molecular weight	847.06
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.24
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	81000
Excitation (nm)	534
Emission (nm)	553
Quantum yield	0.61 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 14, 2025