Name: XFD532-Wheat Germ Agglutinin (WGA) Conjugate
Brand: AAT Bioquest
SKU: 25502
Price: 111 USD
Availability: InStock

XFD532-Wheat Germ Agglutinin (WGA) Conjugate

XFD532, manufactured by AAT Bioquest, has a similar chemical structure to Alexa Fluor® 532, a trademark of Thermo Fisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives, and conjugates are widely used to label cell membranes and fibrotic scar tissue for fluorescence imaging and analysis. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD532 WGA conjugate, like its counterpart Alexa Fluor® 532 WGA conjugate, emits a bright yellow fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25502	1 mg	Price

References

View all 4 references: Citation Explorer

Fluorescence quenching of tryptophan by trifluoroacetamide.

Authors:

Midoux, P and Wahl, P and Auchet, J C and Monsigny, M

Journal:

Biochimica et biophysica acta (1984): 16-25

The binding of monosaccharides to wheat germ agglutinin: fluorescence and NMR investigations.

Authors:

Midoux, P and Grivet, J P and Delmotte, F and Monsigny, M

Journal:

Biochemical and biophysical research communications (1984): 603-11

Localization of chitin in the cell wall of Candida albicans by means of wheat germ agglutinin. Fluorescence and ultrastructural studies.

Authors:

Tronchin, G and Poulain, D and Herbaut, J and Biguet, J

Journal:

European journal of cell biology (1981): 121-8

Nanosecond-pulse fluorimetry of wheat-germ agglutinin (lectin).

Authors:

Privat, J P and Wahl, P and Monsigny, M and Auchet, J C

Journal:

European journal of biochemistry (1976): 573-80

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.24
Correction factor (280 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	81000
Excitation (nm)	534
Emission (nm)	553
Quantum yield	0.61 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on April 29, 2026