XFD546 amine
Product key features
- Ex/Em: 561/572 nm
- Extinction coefficient: 112,000 cm-1M-1
- Reactive Group: Amine
- Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
- High Quantum Yield & Stability: Exhibits intense fluorescence with resilience to photobleaching, thermal fluctuations, and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Product description
XFD546, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 546 (ThermoFisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4–10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays.
XFD546 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.30 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.43 | 0.56 |
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.21 | 0.12 |
XFD532 amine | 534 | 553 | 81000 | 0.24 | 0.09 |
XFD555 amine | 553 | 568 | 155000 | 0.08 | 0.08 |
XFD568 amine | 579 | 603 | 91300 | 0.45 | 0.46 |
XFD647 amine | 650 | 671 | 270000 | - | 0.03 |
XFD750 amine | 752 | 776 | 290000 | - | 0.04 |
XFD350 amine | 343 | 441 | 19000 | 0.25 | 0.19 |
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