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AAT Bioquest

XFD546 amine

Product key features

  • Ex/Em: 561/572 nm
  • Extinction coefficient: 112,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • High Quantum Yield & Stability: Exhibits intense fluorescence with resilience to photobleaching, thermal fluctuations, and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD546, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 546 (ThermoFisher). It exhibits bright orange fluorescence and is readily excited by laser lines at 488 nm or 532 nm, making it highly suitable for applications such as fluorescence microscopy and flow cytometry. XFD546 demonstrates excellent aqueous solubility and pH-insensitivity over a broad range (pH 4–10), ensuring stable signal generation under varying experimental conditions. Additionally, the dye enables high molar ratio conjugation to proteins with minimal fluorescence quenching, facilitating the generation of brighter conjugates for enhanced detection sensitivity. With its high fluorescence quantum yield and superior photostability, XFD546 is particularly advantageous for detecting low-abundance biological targets, providing researchers with improved sensitivity and precision in quantitative fluorescence-based assays.

XFD546 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.300.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.430.56
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.210.12
XFD532 amine534553810000.240.09
XFD555 amine5535681550000.080.08
XFD568 amine579603913000.450.46
XFD647 amine650671270000-0.03
XFD750 amine752776290000-0.04
XFD350 amine343441190000.250.19
XFD405 amine40142135,0000.230.70
XFD430 amine43254015,000-0.28
XFD514 amine518543800000.310.18
XFD546 TCO5615721120000.210.12
XFD546 Tetrazine5615721120000.210.12
XFD610 amine611629144,0000.430.44
ICG amine789813230000-0.076
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Page updated on December 6, 2024

Ordering information

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Unit size
Catalog Number70053
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

1117.44

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.12

Extinction coefficient (cm -1 M -1)

112000

Excitation (nm)

561

Emission (nm)

572

Quantum yield

0.791

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501