XFD546 tyramide reagent

XFD546 Tyramide Reagent is a orange fluorescent dye with an excitation/emission of 561/572 nm that is used for flow cytometry and microscopy applications.

Tyramide Signal Amplification (TSA): Facilitates ultrasensitive detection of low-abundance targets, ideal for IHC and ICC applications
Bright & Stable XFD546 Dye: Delivers high quantum yield with robust resistance to photobleaching, temperature shifts, and pH variations (4–10)

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11075	200 slides	Price

Overview

XFD546 is manufactured by AAT Bioquest, and it has a chemical structure similar to that of Alexa Fluor® 546 (Alexa Fluor® is the trademark of Thermo Fisher). For many immunohistochemical (IHC) applications, the traditional enzymatic amplification procedures are sufficient for achieving adequate antigen detection. However, several factors limit the sensitivity and utility of these procedures. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP. To achieve maximal IHC detection, tyramine is prelabeled with a fluorophore. The signal amplification conferred by the turnover of multiple tyramide substrates per peroxidase label translates ultrasensitive detection of low-abundance targets and the use of smaller amounts of antibodies and hybridization probes. In immunohistochemical applications, sensitivity enhancements derived from TSA method allow primary antibody dilutions to be increased to reduce nonspecific background signals, and can overcome weak immunolabeling caused by suboptimal fixation procedures or low levels of target expression. XFD546 tyramide contains the bright XFD546 dye that can be readily detected with the standard TRITC filter set.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD546 tyramide reagent to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	77.95 µL	389.748 µL	779.496 µL	3.897 mL	7.795 mL
5 mM	15.59 µL	77.95 µL	155.899 µL	779.496 µL	1.559 mL
10 mM	7.795 µL	38.975 µL	77.95 µL	389.748 µL	779.496 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1282.88 g/mol	=	mL	x	mM	=	mmol

Citations

View all 16 citations: Citation Explorer

Dynamic regulation of proximal tubular autophagy from injury to repair after ischemic kidney damage

Authors:

Gong, Yuhong and Zhu, Wei and Li, Yongqiang and Lu, Tao and Tan, Jiexing and He, Changsheng and Yang, Luodan and Zhu, Yufeng and Gong, Li

Journal:

Cellular \& Molecular Biology Letters (2024): 151

Electroretinography Changes in Feline Model of Iodoacetic Acid-induced Retinal Degeneration

Authors:

Kim, Changzoo and Lee, Myungshin and Lee, Seung Uk and Lee, Sang Joon

Journal:

Journal of the Korean Ophthalmological Society (2019): 1205--1215

An ultrasensitive electrochemical immunosensor for procalcitonin detection based on the gold nanoparticles-enhanced tyramide signal amplification strategy

Authors:

Liu, P., Li, C., Zhang, R., Tang, Q., Wei, J., Lu, Y., Shen, P.

Journal:

Biosens Bioelectron (2019): 543-550

A amperometric immunosensor for sensitive detection of circulating tumor cells using a tyramide signal amplification-based signal enhancement system

Authors:

Zhou, X., Li, Y., Wu, H., Huang, W., Ju, H., Ding, S.

Journal:

Biosens Bioelectron (2019): 88-94

Gold nanoparticle labeling with tyramide signal amplification for highly sensitive detection of alpha fetoprotein in human serum by ICP-MS

Authors:

Li, X., Chen, B., He, M., Xiao, G., Hu, B.

Journal:

Talanta (2018): 40-46

References

View all 74 references: Citation Explorer

Tyramide Signal Amplification for Immunofluorescent Enhancement

Authors:

Faget L, Hnasko TS.

Journal:

Methods Mol Biol (2015): 161

Enhanced detection of Porcine reproductive and respiratory syndrome virus in fixed tissues by in situ hybridization following tyramide signal amplification

Authors:

Trang NT, Hirai T, Ngan PH, Lan NT, Fuke N, Toyama K, Yamamoto T, Yamaguchi R.

Journal:

J Vet Diagn Invest (2015): 326

Sensitive whole-mount fluorescent in situ hybridization in zebrafish using enhanced tyramide signal amplification

Authors:

Lauter G, Soll I, Hauptmann G.

Journal:

Methods Mol Biol (2014): 175

KSHV cell attachment sites revealed by ultra sensitive tyramide signal amplification (TSA) localize to membrane microdomains that are up-regulated on mitotic cells

Authors:

Garrigues HJ, Rubinchikova YE, Rose TM.

Journal:

Virology (2014): 75

Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification

Authors:

Aydin M, Herzig GP, Jeong KC, Dunigan S, Shah P, Ahn S.

Journal:

J Food Prot (2014): 100

Physical properties

Molecular weight	1282.88
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.12
Extinction coefficient (cm ^-1 M ^-1)	112000
Excitation (nm)	561
Emission (nm)	572
Quantum yield	0.79 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 12, 2025