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XFD555 acid

XFD555 acid is the same molecule to the Alexa Fluor® 555 acid. It is a cyanine dye with bright yellow-fluorescent dye optimal for labeling antibodies. XFD555 acid is the most economic amine-reactive form for conjugating this dye to a peptide.
XFD555 acid is the same molecule to the Alexa Fluor® 555 acid. It is a cyanine dye with bright yellow-fluorescent dye optimal for labeling antibodies. XFD555 acid is the most economic amine-reactive form for conjugating this dye to a peptide.
XFD555 acid is the same molecule to the Alexa Fluor® 555 acid. It is a cyanine dye with bright yellow-fluorescent dye optimal for labeling antibodies. XFD555 acid is the most economic amine-reactive form for conjugating this dye to a peptide.
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Physical properties
Molecular weight946.99
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.08
Correction Factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)155000
Excitation (nm)553
Emission (nm)568
Quantum yield0.11
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
XFD555 amine
Related products
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
XFD532 amine
XFD568 amine
XFD647 amine
XFD750 amine
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]
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OverviewpdfSDSpdfProtocol


Molecular weight
946.99
Correction Factor (260 nm)
0.08
Correction Factor (280 nm)
0.08
Extinction coefficient (cm -1 M -1)
155000
Excitation (nm)
553
Emission (nm)
568
Quantum yield
0.11
XFD555 acid is the same molecule to the Alexa Fluor® 555 acid (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright yellow-fluorescent dye optimal for labeling antibodies. XFD555 dye is water soluble and pH-insensitive from pH 4 to 10. XFD555 acid is the most economic amine-reactive form for conjugating this dye to a peptide.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD555 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM105.598 µL527.989 µL1.056 mL5.28 mL10.56 mL
5 mM21.12 µL105.598 µL211.195 µL1.056 mL2.112 mL
10 mM10.56 µL52.799 µL105.598 µL527.989 µL1.056 mL

Molarity calculator

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Spectrum


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Spectral properties

Correction Factor (260 nm)0.08
Correction Factor (280 nm)0.08
Extinction coefficient (cm -1 M -1)155000
Excitation (nm)553
Emission (nm)568
Quantum yield0.11

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.240.09
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.300.11
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.250.19
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.210.12
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.450.46
XFD514 acid51854380000-0.310.18
XFD647 acid6506712700000.331-0.03
XFD750 acid7527762900000.121-0.04
XFD700 acid6967192050000.251-0.07
ICG acid789813230000--0.076
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.070.073
Cy5NS acid65167025000010.271, 0.420.020.03
Cy7NS acid7567792500000.30.050.036
AMCA acid *CAS#: 106562-32-7*34643419000--0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900--0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900--0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
BCECF acid *CAS#: 85138-49-4*50452790000---
Cy3B acid56057112000010.5810.0480.069
QXY7 acid [equivalent to QSY-7 acid]--900001--0.22
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Images


References


View all 50 references: Citation Explorer
Preclinical evaluation and pilot clinical study of [18F]AlF-NOTA-FAPI-04 for PET imaging of rheumatoid arthritis.
Authors: Ge, Luna and Fu, Zheng and Wei, Yuchun and Shi, Dandan and Geng, Yun and Fan, Huancai and Zhang, Ruojia and Zhang, Yuang and Li, Shufeng and Wang, Shijie and Shi, Haojun and Song, Guanhua and Pan, Jihong and Cheng, Kai and Wang, Lin
Journal: European journal of nuclear medicine and molecular imaging (2022): 4025-4036
Enhanced Cellular Uptake in an Electrostatically Interacting Fucoidan-L-Arginine Fiber Complex.
Authors: Arunagiri, Vinothini and Tsai, Hsieh-Chih and Darge, Haile Fentahun and Hanurry, Endiries Yibru and Lee, Chang Yi and Lai, Juin-Yih and Wu, Szu-Yuan
Journal: Polymers (2021)
switchSENSE Technology for Analysis of DNA Polymerase Kinetics.
Authors: Bec, Guillaume and Ennifar, Eric
Journal: Methods in molecular biology (Clifton, N.J.) (2021): 145-153
Accurate Modeling of Excitonic Coupling in Cyanine Dye Cy3.
Authors: Sorour, Mohammed I and Kistler, Kurt A and Marcus, Andrew H and Matsika, Spiridoula
Journal: The journal of physical chemistry. A (2021): 7852-7866
A Fluorescence and Surface-Enhanced Raman Spectroscopic Dual-Modal Aptasensor for Sensitive Detection of Cyanotoxins.
Authors: Li, Ming and Lin, Hangduo and Paidi, Santosh Kumar and Mesyngier, Nicolas and Preheim, Sarah and Barman, Ishan
Journal: ACS sensors (2020): 1419-1426
The synthetic steroid tibolone exerts sex-specific regulation of astrocyte phagocytosis under basal conditions and after an inflammatory challenge.
Authors: Crespo-Castrillo, Andrea and Garcia-Segura, Luis-Miguel and Arevalo, Maria-Angeles
Journal: Journal of neuroinflammation (2020): 37
Single-molecule chemistry. Part I: monitoring oxidation of G in oligonucleotides using CY3 fluorescence.
Authors: Sobek, Jens and Schmidt, Marco and Grossmann, Jonas and Rehrauer, Hubert and Schmidt, Lucas and Schlapbach, Ralph
Journal: Methods and applications in fluorescence (2020): 035010
The effect of structural heterogeneity upon the microviscosity of ionic liquids.
Authors: Clark, Ryan and Nawawi, Mohd A and Dobre, Ana and Pugh, David and Liu, Qingshan and Ivanov, Aleksandar P and White, Andrew J P and Edel, Joshua B and Kuimova, Marina K and McIntosh, Alastair J S and Welton, Tom
Journal: Chemical science (2020): 6121-6133
Excimer-FRET Cascade in Dual DNA Probes: Open Access to Large Stokes Shift, Enhanced Acceptor Light up, and Robust RNA Sensing.
Authors: Aparin, Ilya O and Sergeeva, Olga V and Mishin, Alexander S and Khaydukov, Evgeny V and Korshun, Vladimir A and Zatsepin, Timofei S
Journal: Analytical chemistry (2020)
The dual-function of lipoic acid groups as surface anchors and sulfhydryl reactive sites on polymer-stabilized QDs and Au nanocolloids.
Authors: Jin, Zhicheng and Kapur, Anshika and Wang, Wentao and Diaz Hernandez, Juan and Thakur, Mannat and Mattoussi, Hedi
Journal: The Journal of chemical physics (2019): 164703