XFD555 amine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1103.10 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.08 |
| Extinction coefficient (cm -1 M -1) | 155000 |
| Excitation (nm) | 553 |
| Emission (nm) | 568 |
| Quantum yield | 0.11 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Direct upgrades
| iFluor® 555 amine |
Alternative formats
| XFD555 acid |
Related products
| Overview |  SDSProtocol |
Upgrade: iFluor® 555 amine
Molecular weight 1103.10 | Correction Factor (260 nm) 0.08 | Correction Factor (280 nm) 0.08 | Extinction coefficient (cm -1 M -1) 155000 | Excitation (nm) 553 | Emission (nm) 568 | Quantum yield 0.11 |
XFD555 amine is the same molecule to the Alexa Fluor® 555 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright yellow-fluorescence optimal for labeling antibodies. XFD555 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD555 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD555 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 90.654 µL | 453.268 µL | 906.536 µL | 4.533 mL | 9.065 mL |
| 5 mM | 18.131 µL | 90.654 µL | 181.307 µL | 906.536 µL | 1.813 mL |
| 10 mM | 9.065 µL | 45.327 µL | 90.654 µL | 453.268 µL | 906.536 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.08 |
| Correction Factor (280 nm) | 0.08 |
| Extinction coefficient (cm -1 M -1) | 155000 |
| Excitation (nm) | 553 |
| Emission (nm) | 568 |
| Quantum yield | 0.11 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 490 | 520 | 71000 | 0.921 | 0.11 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 375 | 618 | 90000 | 0.661 | 0.56 |
| XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.09 |
| XFD568 amine | 579 | 603 | 88000 | 0.691 | 0.46 |
| XFD647 amine | 650 | 671 | 270000 | 0.331 | 0.03 |
| XFD750 amine | 752 | 776 | 290000 | 0.121 | 0.04 |
| ICG amine | 789 | 814 | - | - | 0.076 |
Images
Figure 1. XFD555 amine is the same molecule to the Alexa Fluor® 555 amine. It is a cyanine dye with bright yellow-fluorescence optimal for labeling antibodies.
References
View all 50 references: Citation Explorer
Exclusion of Anchor-Matched Peptide Nucleic Acid from Liquid-Ordered Domains by Hybridization with Complementary Flavin-Labeled DNA.
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113
Authors: Oka, Yoshimi
Journal: ACS omega (2023): 1109-1113
High-Efficiency Expression and Purification of DNAJB6b Based on the pH-Modulation of Solubility and Denaturant-Modulation of Size.
Authors: Linse, Sara
Journal: Molecules (Basel, Switzerland) (2022)
Authors: Linse, Sara
Journal: Molecules (Basel, Switzerland) (2022)
Pathogen infection-responsive nanoplatform targeting macrophage endoplasmic reticulum for treating life-threatening systemic infection.
Authors: Zhao, Yan and Liu, Shuo and Shi, Zhishang and Zhu, Hangqi and Li, Mingchun and Yu, Qilin
Journal: Nano research (2022): 6243-6255
Authors: Zhao, Yan and Liu, Shuo and Shi, Zhishang and Zhu, Hangqi and Li, Mingchun and Yu, Qilin
Journal: Nano research (2022): 6243-6255
Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells.
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Alexa Fluor 488-conjugated cholera toxin subunit B optimally labels neurons 3-7 days after injection into the rat gastrocnemius muscle.
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Authors: Cui, Jing-Jing and Wang, Jia and Xu, Dong-Sheng and Wu, Shuang and Guo, Ya-Ting and Su, Yu-Xin and Liu, Yi-Han and Wang, Yu-Qing and Jing, Xiang-Hong and Bai, Wan-Zhu
Journal: Neural regeneration research (2022): 2316-2320
Another Wrinkle with Age: Aged Collagen and Intra-peritoneal Metastasis of Ovarian Cancer.
Authors: Harper, Elizabeth I and Hilliard, Tyvette S and Sheedy, Emma F and Carey, Preston and Wilkinson, Paul and Siroky, Michael D and Yang, Jing and Agadi, Elizabeth and Leonard, Annemarie K and Low, Ethan and Liu, Yueying and Biragyn, Arya and Annunziata, Christina M and Stack, M Sharon
Journal: Aging and cancer (2022): 116-129
Authors: Harper, Elizabeth I and Hilliard, Tyvette S and Sheedy, Emma F and Carey, Preston and Wilkinson, Paul and Siroky, Michael D and Yang, Jing and Agadi, Elizabeth and Leonard, Annemarie K and Low, Ethan and Liu, Yueying and Biragyn, Arya and Annunziata, Christina M and Stack, M Sharon
Journal: Aging and cancer (2022): 116-129
Apoptosis Detection in Retinal Ganglion Cells Using Quantitative Changes in Multichannel Fluorescence Colocalization.
Authors: Qiu, Xudong and Gammon, Seth T and Johnson, James R and Pisaneschi, Federica and Millward, Steven W and Barnett, Edward M and Piwnica-Worms, David
Journal: Biosensors (2022)
Authors: Qiu, Xudong and Gammon, Seth T and Johnson, James R and Pisaneschi, Federica and Millward, Steven W and Barnett, Edward M and Piwnica-Worms, David
Journal: Biosensors (2022)
A RAC-alpha serine/threonine-protein kinase (CgAKT1) involved in the synthesis of CgIFNLP in oyster Crassostrea gigas.
Authors: Hou, Lilin and Qiao, Xue and Li, Youjing and Jin, Yuhao and Liu, Ranyang and Wang, Sicong and Zhou, Kai and Wang, Lingling and Song, Linsheng
Journal: Fish & shellfish immunology (2022): 129-139
Authors: Hou, Lilin and Qiao, Xue and Li, Youjing and Jin, Yuhao and Liu, Ranyang and Wang, Sicong and Zhou, Kai and Wang, Lingling and Song, Linsheng
Journal: Fish & shellfish immunology (2022): 129-139
pH-responsive graphene oxide loaded with targeted peptide and anticancer drug for OSCC therapy.
Authors: Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie
Journal: Frontiers in oncology (2022): 930920
Authors: Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie
Journal: Frontiers in oncology (2022): 930920
Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells.
Authors: Mazloom-Farsibaf, Hanieh and Farzam, Farzin and Fazel, Mohamadreza and Wester, Michael J and Meddens, Marjolein B M and Lidke, Keith A
Journal: PloS one (2021): e0246138
Authors: Mazloom-Farsibaf, Hanieh and Farzam, Farzin and Fazel, Mohamadreza and Wester, Michael J and Meddens, Marjolein B M and Lidke, Keith A
Journal: PloS one (2021): e0246138