XFD555 amine

• Ex/Em: 553/568 nm
• Extinction coefficient: 150,000 cm^-1M^-1
• Reactive Group: Amine
• Versatile Conjugation: Efficiently labels carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
• Bright & Stable: Exhibits intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
• Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

XFD555, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 555 (ThermoFisher). It is a bright orange-fluorescent dye with an excitation optimized for use with either the 488 nm line of the argon-ion laser or the 532 nm line of the frequency-doubled Nd:YAG laser. The high fluorescence quantum yield and high photostability of XFD555 allow for the detection of low-abundance biological structures with great sensitivity. XFD555 demonstrates good aqueous solubility and pH-insensitivity over a broad pH range (pH 4–10), ensuring stable fluorescence generation under varying experimental conditions. XFD555 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection in imaging and flow cytometry.

XFD555 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.