XFD594 acid
Product key features
- Ex/Em: 590/618 nm
- Extinction coefficient: 90,000 cm-1M-1
- Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
- Advanced Imaging: Suited for multicolor fluorescence microscopy, flow cytometry, and super-resolution imaging
Product description
XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.
XFD594 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD594 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.661 | 0.43 | 0.56 |
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
XFD514 acid | 518 | 543 | 80000 | - | 0.31 | 0.18 |
XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
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