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AAT Bioquest

XFD594 acid

Product key features

  • Ex/Em: 590/618 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
  • Advanced Imaging: Suited for multicolor fluorescence microscopy, flow cytometry, and super-resolution imaging

Product description

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

XFD594 acid is a non-reactive compound that can be employed as a reference standard in studies utilizing XFD594 conjugates. It is also suitable for use as a control in confocal microscopy, immunocytochemistry (ICC), high-content screening (HCS), flow cytometry, and live cell imaging applications. Furthermore, it can be utilized in the synthesis of activated esters and STP and can be coupled to hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides (e.g., EDAC). This allows for the conjugation of the dye to amino-containing molecules, such as proteins, antibodies, amine-modified oligonucleotides, and peptides.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.240.09
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.300.11
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.250.19
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.210.12
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.450.46
XFD514 acid51854380000-0.310.18
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.331-0.03
XFD750 acid7527762900000.121-0.04
XFD700 acid6967192050000.251-0.07
XFD405 acid40142135,000-0.230.70
XFD430 acid43254015,000--0.28
XFD594 TCO590618900000.6610.430.56
XFD594 Tetrazine590618900000.6610.430.56
XFD610 acid611629144,000-0.430.44
ICG acid789813230000--0.076
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.070.073
Cy5NS acid65167025000010.271, 0.420.020.03
Cy7NS acid7567792500000.30.050.036
AMCA acid *CAS#: 106562-32-7*34643419000--0.153
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900--0.107
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900--0.107
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*-----0.516
BCECF acid *CAS#: 85138-49-4*50452790000---
Cy3B acid56057112000010.5810.0480.069
QXY7 acid [equivalent to QSY-7 acid]--900001--0.22
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000--0.19
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Page updated on December 11, 2024

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Catalog Number1794
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Physical properties

Molecular weight

694.73

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501