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AAT Bioquest

XFD594 alkyne

Product key features

  • Ex/Em: 590/618 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive Group: Alkyne
  • Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

The alkyne derivative of XFD594 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*499520710000.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.56
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.56
XFD647 Alkyne6506712700000.03
XFD350 alkyne343441190000.19
XFD405 alkyne40142135,0000.70
XFD430 alkyne43254015,0000.28
XFD514 alkyne518543800000.18
XFD546 alkyne5615721120000.12
XFD532 alkyne534553810000.09
XFD568 alkyne579603913000.46
XFD594 TCO590618900000.56
XFD594 Tetrazine590618900000.56
XFD610 alkyne611629144,0000.44
AMCA Alkyne346434190000.153
ICG alkyne7898132300000.076
Cy3B alkyne56057112000010.069
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Page updated on December 11, 2024

Ordering information

Price
Unit size
Catalog Number1730
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Physical properties

Molecular weight

759.85

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501