XFD594 alkyne
Product key features
- Ex/Em: 590/618 nm
- Extinction coefficient: 90,000 cm-1M-1
- Reactive Group: Alkyne
- Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
- Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
- Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies
Product description
XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.
The alkyne derivative of XFD594 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.
Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.56 |
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594* | 590 | 618 | 92000 | 0.56 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
XFD350 alkyne | 343 | 441 | 19000 | 0.19 |
XFD405 alkyne | 401 | 421 | 35,000 | 0.70 |
XFD430 alkyne | 432 | 540 | 15,000 | 0.28 |
XFD514 alkyne | 518 | 543 | 80000 | 0.18 |
XFD546 alkyne | 561 | 572 | 112000 | 0.12 |
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