logo
AAT Bioquest

XFD594 PEG4 DBCO

Product key features

  • Ex/Em: 590/618 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive Group: DBCO
  • Copper-Free Bioorthogonal Labeling: Enables rapid and efficient azide conjugation via copper-free SPAAC
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic PEG4 Spacer: Enhances solubility and minimizes steric hindrance for enhanced biocompatibility

Product description

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

The DBCO derivative of XFD594 is a highly reactive cycloalkyne optimized for copper-free click chemistry (SPAAC, strain-promoted azide-alkyne cycloaddition). This derivative exhibits a significantly higher reaction rate with azides compared to other cyclooctynes and copper-catalyzed click reactions (CuAAC). Uniquely, DBCO does not react with tetrazines, allowing for its use in bioorthogonal reactions alongside trans-cyclooctenes and tetrazines. For applications where the presence of copper is problematic, XFD594 DBCO serves as an effective alternative to copper-dependent fluorescent alkynes.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
XFD488 PEG4 DBCO499520710000.300.11
XFD350 PEG4 DBCO343441190000.250.19
XFD405 PEG4 DBCO40142135,0000.230.70
XFD430 PEG4 DBCO43254015,000-0.28
XFD514 PEG4 DBCO518543800000.310.18
XFD546 PEG4 DBCO5615721120000.210.12
XFD532 PEG4 DBCO534553810000.240.09
XFD568 PEG4 DBCO579603913000.450.46
XFD610 PEG4 DBCO611629144,0000.430.44
Page updated on December 12, 2024

Ordering information

Price
Unit size
Catalog Number1731
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1228.40

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501