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AAT Bioquest

XFD594 TCO

Product key features

  • Ex/Em: 590/618 nm
  • Extinction coefficient: 90,000 cm-1M-1
  • Reactive Group: TCO
  • Click Chemistry Conjugation: Provides rapid and copper-free labeling of tetrazine-modified biomolecules
  • Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
  • Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Product description

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

XFD594 TCO is particularly useful for labeling tetrazine-modified biomolecules under copper-free conditions. It reacts with tetrazine-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*590618920000.6610.430.56
XFD488 TCO499520730000.9210.30.11
XFD350 TCO34344119000-0.250.19
XFD405 TCO40142135,000-0.230.70
XFD430 TCO43254015,000--0.28
XFD514 TCO51854380000-0.310.18
XFD546 TCO5615721120000.7910.210.12
XFD532 TCO534553810000.6110.240.09
XFD568 TCO579603913000.6910.450.46
XFD594 acid590618900000.6610.430.56
XFD594 azide590618900000.6610.430.56
XFD594 alkyne590618900000.6610.430.56
XFD594 Tetrazine590618900000.6610.430.56
XFD610 TCO611629144,000-0.430.44
Cy3B TCO56057112000010.5810.0480.069
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Page updated on December 11, 2024

Ordering information

Price
Unit size
Catalog Number1732
Quantity
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Molecular weight

931.09

Solvent

DMSO

Spectral properties

Absorbance (nm)

592

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.56

Extinction coefficient (cm -1 M -1)

90000

Excitation (nm)

590

Emission (nm)

618

Quantum yield

0.661

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501