XFD594 Tetrazine

• Ex/Em: 590/618 nm
• Extinction coefficient: 90,000 cm^-1M^-1
• Reactive Group: Tetrazine
• Click Chemistry Conjugation: Provides rapid, copper-free labeling of TCO-modified biomolecules
• Bright & Stable: Provides high quantum yield with resilience to photobleaching and pH variations from 4 to 10
• Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

XFD594, manufactured by AAT Bioquest, is structurally identical to Alexa Fluor™ 594 (ThermoFisher). This bright red-fluorescent dye is efficiently excited by the 561 nm or 594 nm laser lines and is compatible with RFP filters like Texas Red. It demonstrates excellent solubility in aqueous solutions and is pH-insensitive across a broad range (pH 4–10), ensuring reliable and stable signal generation under diverse experimental conditions. XFD594 is particularly well-suited for multicolor fluorescence microscopy, flow cytometry, and advanced SRM imaging techniques like dSTORM, SIM, STED and TPE. It can be conjugated to proteins at high molar ratios with minimal self-quenching, resulting in brighter conjugates. Moreover, the superior fluorescence quantum yield and photostability of XFD594 make it ideal for detecting low-abundance biological targets, enabling greater precision and sensitivity in quantitative fluorescence assays.

XFD594 tetrazine is particularly useful for labeling TCO-modified biomolecules under copper-free conditions. It reacts with TCO-functionalized molecules, forming a stable conjugate via a dihydropyrazine moiety. This click reaction is favored over others due to its extremely fast kinetics and higher yields under mild reaction conditions, making it a popular choice for researchers.