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AAT Bioquest

XFD610 alkyne

Product key features

  • Ex/Em: 611/629 nm
  • Extinction coefficient: 144,000 cm-1M-1
  • Reactive Group: Alkyne
  • Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
  • Advanced Dye Properties: Excellent water solubility and superior optical properties to Texas Red™; structural match to Alexa Fluor™ 610
  • Robust Signal Performance: Maintains high intensity and pH independence (pH 4-10) with exceptional photostability

Product description

XFD610, manufactured by AAT Bioquest, is a red-fluorescent dye that is structurally identical to Alexa Fluor™ 610 (ThermoFisher). The dye exhibits high photostability and strong fluorescence, making it well-suited for advanced imaging applications and flow cytometry. Its water solubility and pH-independent fluorescence over a broad range (pH 4–10) ensure consistent performance under diverse experimental conditions. XFD610 enables reproducible labeling with high signal intensity, providing reliable results in fluorescence-based assays. Additionally, it serves as a robust alternative to Texas Red™, offering improved optical properties for experiments requiring enhanced fluorescence output.

The alkyne derivative of XFD610 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.

Spectrum

Page updated on April 15, 2025

Ordering information

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Unit size
Catalog Number70065
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
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Physical properties

Molecular weight

1022.42

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.43

Correction Factor (280 nm)

0.44

Extinction coefficient (cm -1 M -1)

144,000

Excitation (nm)

611

Emission (nm)

629

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Product Image
Product Image
Gallery Image 1
Schematic illustrating the azide–alkyne cycloaddition (“click chemistry”) between an alkyne‐functionalized dye and an azide‐presenting biomolecule. In the presence of a copper catalyst (CuAAC) or under strain‐promoted conditions (SPAAC), the azide and alkyne react to form a stable 1,2,3‐triazole linkage. This highly selective and robust reaction proceeds under mild conditions, tolerates a wide range of functional groups, and is frequently used to label proteins, nucleic acids, and other biomolecules for imaging, proteomics, and high‐throughput assays.