XFD633 alkyne
Product key features
- Ex/Em: 632/650 nm
- Extinction Coefficient: 159,000 cm-1M-1
- Reactive Group: Alkyne
- Efficient Conjugation: Click chemistry labeling of azides on peptides, antibodies, and other biomolecules
- Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 633
- Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability
Product description
XFD633, manufactured by AAT Bioquest, is a bright red-fluorescent dye structurally identical to Alexa Fluor™ 633 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD633 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.
The alkyne derivative of XFD633 is widely used for labeling azides on peptides, antibodies, and other biomolecules via click chemistry. It participates in copper-catalyzed azide-alkyne cycloaddition (CuAAC) with azide-containing molecules.