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AAT Bioquest

XFD633 amine

Product key features

  • Ex/Em: 632/650 nm
  • Extinction Coefficient: 159,000 cm-1M-1
  • Reactive Group: Amine
  • Versatile Conjugation: Efficiently tags carbonyl groups and also serves as an amine donor for enzymatic transamination labeling
  • Advanced Dye Properties: Excellent water solubility and structural match to Alexa Fluor™ 633
  • Robust Signal Performance: Maintains high intensity and pH independence (pH 4-11) with exceptional photostability

Product description

XFD633, manufactured by AAT Bioquest, is a bright red-fluorescent dye structurally identical to Alexa Fluor™ 633 (ThermoFisher). It is characterized by its intense brightness, high fluorescence quantum yield and photostability. The dye demonstrates excellent solubility in aqueous solutions and retains pH-independent fluorescence over a broad range (pH 4–11), ensuring consistent performance across diverse experimental conditions. Optimally excited by the 633 nm emission line of He-Ne lasers or the 635 nm diode laser, XFD633 is particularly well-suited for flow cytometry. Its robust and uniform labeling properties yield high signal intensity and reproducibility, making it an ideal choice for advanced fluorescence imaging, flow cytometry, and various fluorescence-based analytical techniques.

XFD633 amine is a carbonyl-reactive building block for modifying carboxylic groups in the presence of activators such as EDC or DCC, or activated esters like NHS esters, through the formation of stable amide bonds. Additionally, it can be used as an amine donor for enzymatic transamination labeling.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD350 amine34344119000-0.250.19
XFD405 amine40142135,000-0.230.70
XFD430 amine43254015,000--0.28
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.300.11
XFD514 amine51854380000-0.310.18
XFD532 amine534553810000.6110.240.09
XFD546 amine5615721120000.7910.210.12
XFD555 amine5535681550000.110.080.08
XFD568 amine579603913000.6910.450.46
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD610 amine611629144,000-0.430.44
XFD633 TCO632650159,000--0.55
XFD633 Tetrazine632650159,000--0.55
XFD635 amine633647140,000---
XFD647 amine6506712700000.331-0.03
XFD660 amine663691132000-0.000.10
XFD680 amine6817041840000.3610.000.05
XFD700 amine6967191920000.2510.000.07
XFD750 amine7527762900000.121-0.04
XFD790 amine782805260,000-0.090.08
Show More (11)
Page updated on May 19, 2025

Ordering information

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Catalog Number70073
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Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.55

Extinction coefficient (cm -1 M -1)

159,000

Excitation (nm)

632

Emission (nm)

650

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.
Schematic illustrating the carboxyl–amine coupling between an amine‐functionalized dye and a carboxyl‐modified biomolecule. In practice, carbodiimide reagents (e.g., EDC) are often used to activate carboxyl groups, facilitating subsequent amide bond formation with primary amines. This versatile approach enables conjugation of peptides, proteins, and nucleic acids that possess free carboxyl or amine functionalities.