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XFD647 acid
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 973.03 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| XFD647 amine |
| XFD647 Azide |
| XFD647 Alkyne |
Related products
| Overview |  SDSProtocol |
See also: Antibody and Protein Labeling
Molecular weight 973.03 | Correction Factor (280 nm) 0.03 | Correction Factor (565 nm) 0.1109 | Extinction coefficient (cm -1 M -1) 270000 | Excitation (nm) 650 | Emission (nm) 671 | Quantum yield 0.331 |
XFD647 acid is the same molecule to the Alexa Fluor® 647 acid (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy5 dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to 10. XFD647 acid is the most economic amine-reactive form for conjugating this dye to a peptide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 102.772 µL | 513.859 µL | 1.028 mL | 5.139 mL | 10.277 mL |
| 5 mM | 20.554 µL | 102.772 µL | 205.544 µL | 1.028 mL | 2.055 mL |
| 10 mM | 10.277 µL | 51.386 µL | 102.772 µL | 513.859 µL | 1.028 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD488 acid *Same Structure to Alexa Fluor™ 488 acid* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 acid *Same Structure to Alexa Fluor™ 350 acid* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD546 acid *Same Structure to Alexa Fluor™ 546 acid* | 561 | 572 | 112000 | 0.791 | 0.21 | 0.12 |
| XFD568 acid *Same Structure to Alexa Fluor™ 568 acid* | 579 | 603 | 91300 | 0.691 | 0.45 | 0.46 |
| XFD514 acid | 518 | 543 | 80000 | - | 0.31 | 0.18 |
| XFD555 acid | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD750 acid | 752 | 776 | 290000 | 0.121 | - | 0.04 |
| XFD700 acid | 696 | 719 | 205000 | 0.251 | - | 0.07 |
Show More (13) | ||||||
Images
Figure 1. XFD647 acid is the same molecule to the Alexa Fluor® 647 acid. It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy5 dye.
References
View all 50 references: Citation Explorer
Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Cranial Bone Transport Promotes Angiogenesis, Neurogenesis, and Modulates Meningeal Lymphatic Function in Middle Cerebral Artery Occlusion Rats.
Authors: Bai, Shanshan and Lu, Xuan and Pan, Qi and Wang, Bin and Pong U, Kin and Yang, Yongkang and Wang, Haixing and Lin, Sien and Feng, Lu and Wang, Yan and Li, Yucong and Lin, Weiping and Wang, Yujia and Zhang, Xiaoting and Li, Yuan and Li, Linlong and Yang, Zhengmeng and Wang, Ming and Lee, Wayne Yuk-Wai and Jiang, Xiaohua and Li, Gang
Journal: Stroke (2022): 1373-1385
Authors: Bai, Shanshan and Lu, Xuan and Pan, Qi and Wang, Bin and Pong U, Kin and Yang, Yongkang and Wang, Haixing and Lin, Sien and Feng, Lu and Wang, Yan and Li, Yucong and Lin, Weiping and Wang, Yujia and Zhang, Xiaoting and Li, Yuan and Li, Linlong and Yang, Zhengmeng and Wang, Ming and Lee, Wayne Yuk-Wai and Jiang, Xiaohua and Li, Gang
Journal: Stroke (2022): 1373-1385
Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.
Authors: Saavedra, Lucas A and Buena-Maizón, Héctor and Barrantes, Francisco J
Journal: International journal of molecular sciences (2022)
Authors: Saavedra, Lucas A and Buena-Maizón, Héctor and Barrantes, Francisco J
Journal: International journal of molecular sciences (2022)
Cell-based flow cytometry assay for simultaneous detection of multiple autoantibodies in a single serum sample.
Authors: Tanimura, Yukihiro and Hiroaki, Yoko and Mori, Masahiro and Fujiyoshi, Yoshinori
Journal: Analytical biochemistry (2022): 114721
Authors: Tanimura, Yukihiro and Hiroaki, Yoko and Mori, Masahiro and Fujiyoshi, Yoshinori
Journal: Analytical biochemistry (2022): 114721
Determination of association equilibrium constant from single molecule fluorescence localization microscopy.
Authors: Cappellari, M Victoria and Marcano-García, Luis F and Simoncelli, Sabrina and Aramendía, Pedro F
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 1751-1760
Authors: Cappellari, M Victoria and Marcano-García, Luis F and Simoncelli, Sabrina and Aramendía, Pedro F
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 1751-1760
3D Single Molecule Super-Resolution Microscopy of Whole Nuclear Lamina.
Authors: Rozario, Ashley M and Morey, Alison and Elliott, Cade and Russ, Brendan and Whelan, Donna R and Turner, Stephen J and Bell, Toby D M
Journal: Frontiers in chemistry (2022): 863610
Authors: Rozario, Ashley M and Morey, Alison and Elliott, Cade and Russ, Brendan and Whelan, Donna R and Turner, Stephen J and Bell, Toby D M
Journal: Frontiers in chemistry (2022): 863610
Organotropic dendrons with high potency as HIV-1, HIV-2 and EV-A71 cell entry inhibitors.
Authors: Martí-Marí, Olaia and Martínez-Gualda, Belén and Fernández-Barahona, Irene and Mills, Alberto and Abdelnabi, Rana and Noppen, Sam and Neyts, Johan and Schols, Dominique and Camarasa, María-José and Herranz, Fernando and Gago, Federico and San-Félix, Ana
Journal: European journal of medicinal chemistry (2022): 114414
Authors: Martí-Marí, Olaia and Martínez-Gualda, Belén and Fernández-Barahona, Irene and Mills, Alberto and Abdelnabi, Rana and Noppen, Sam and Neyts, Johan and Schols, Dominique and Camarasa, María-José and Herranz, Fernando and Gago, Federico and San-Félix, Ana
Journal: European journal of medicinal chemistry (2022): 114414
Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells.
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Bivalent EGFR-Targeting DARPin-MMAE Conjugates.
Authors: Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M
Journal: International journal of molecular sciences (2022)
Authors: Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M
Journal: International journal of molecular sciences (2022)
Application notes
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Buccutite™ Bioconjugation Technology
FAQ
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Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?