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XFD647 amine

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Physical properties
Molecular weight1129.14
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.03
Correction Factor (565 nm)0.1109
Extinction coefficient (cm -1 M -1)270000
Excitation (nm)650
Emission (nm)671
Quantum yield0.331
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Direct upgrades
iFluor® 647 amine
Related products
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD488 TCO
XFD555 acid
XFD750 acid
XFD700 acid
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]
Show More (44)

OverviewpdfSDSpdfProtocol


Molecular weight
1129.14
Correction Factor (280 nm)
0.03
Correction Factor (565 nm)
0.1109
Extinction coefficient (cm -1 M -1)
270000
Excitation (nm)
650
Emission (nm)
671
Quantum yield
0.331
XFD647 amine is the same molecule to the Alexa Fluor® 647 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy5 dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD647 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM88.563 µL442.815 µL885.63 µL4.428 mL8.856 mL
5 mM17.713 µL88.563 µL177.126 µL885.63 µL1.771 mL
10 mM8.856 µL44.281 µL88.563 µL442.815 µL885.63 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.03
Correction Factor (565 nm)0.1109
Extinction coefficient (cm -1 M -1)270000
Excitation (nm)650
Emission (nm)671
Quantum yield0.331

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.300.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD532 amine534553810000.6110.240.09
XFD555 amine5535681550000.110.080.08
XFD568 amine579603880000.6910.450.46
XFD750 amine7527762900000.121-0.04
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*6506712390000.3310.000.03
ICG amine789813230000--0.076

Images


References


View all 50 references: Citation Explorer
Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Cranial Bone Transport Promotes Angiogenesis, Neurogenesis, and Modulates Meningeal Lymphatic Function in Middle Cerebral Artery Occlusion Rats.
Authors: Bai, Shanshan and Lu, Xuan and Pan, Qi and Wang, Bin and Pong U, Kin and Yang, Yongkang and Wang, Haixing and Lin, Sien and Feng, Lu and Wang, Yan and Li, Yucong and Lin, Weiping and Wang, Yujia and Zhang, Xiaoting and Li, Yuan and Li, Linlong and Yang, Zhengmeng and Wang, Ming and Lee, Wayne Yuk-Wai and Jiang, Xiaohua and Li, Gang
Journal: Stroke (2022): 1373-1385
Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.
Authors: Saavedra, Lucas A and Buena-Maizón, Héctor and Barrantes, Francisco J
Journal: International journal of molecular sciences (2022)
Cell-based flow cytometry assay for simultaneous detection of multiple autoantibodies in a single serum sample.
Authors: Tanimura, Yukihiro and Hiroaki, Yoko and Mori, Masahiro and Fujiyoshi, Yoshinori
Journal: Analytical biochemistry (2022): 114721
Determination of association equilibrium constant from single molecule fluorescence localization microscopy.
Authors: Cappellari, M Victoria and Marcano-García, Luis F and Simoncelli, Sabrina and Aramendía, Pedro F
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 1751-1760
3D Single Molecule Super-Resolution Microscopy of Whole Nuclear Lamina.
Authors: Rozario, Ashley M and Morey, Alison and Elliott, Cade and Russ, Brendan and Whelan, Donna R and Turner, Stephen J and Bell, Toby D M
Journal: Frontiers in chemistry (2022): 863610
Organotropic dendrons with high potency as HIV-1, HIV-2 and EV-A71 cell entry inhibitors.
Authors: Martí-Marí, Olaia and Martínez-Gualda, Belén and Fernández-Barahona, Irene and Mills, Alberto and Abdelnabi, Rana and Noppen, Sam and Neyts, Johan and Schols, Dominique and Camarasa, María-José and Herranz, Fernando and Gago, Federico and San-Félix, Ana
Journal: European journal of medicinal chemistry (2022): 114414
Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells.
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Bivalent EGFR-Targeting DARPin-MMAE Conjugates.
Authors: Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M
Journal: International journal of molecular sciences (2022)