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XFD647 amine
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Application notes
XFD647 amine is the same molecule to the Alexa Fluor® 647 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy5 dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD647 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD555 amine | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD568 amine | 579 | 603 | 88000 | 0.691 | 0.45 | 0.46 |
| XFD750 amine | 752 | 776 | 290000 | 0.121 | - | 0.04 |
| XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
| ICG amine | 789 | 813 | 230000 | - | - | 0.076 |
References
View all 50 references: Citation Explorer
Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
In Vivo Evaluation of Sgc8-c Aptamer as a Molecular Imaging Probe for Colon Cancer in a Mouse Xenograft Model.
Authors: Arévalo, Ana Paula and Castelli, Romina and Ibarra, Manuel and Crispo, Martina and Calzada, Victoria
Journal: International journal of molecular sciences (2022)
Authors: Arévalo, Ana Paula and Castelli, Romina and Ibarra, Manuel and Crispo, Martina and Calzada, Victoria
Journal: International journal of molecular sciences (2022)
A 5-color flow cytometry panel to assess plasma membrane integrity, acrosomal status, membrane lipid organization and mitochondrial activity of boar and stallion spermatozoa following liquid semen storage.
Authors: Quirino, Monike and Jakop, Ulrike and Mellagi, Ana Paula Gonçalves and Bortolozzo, Fernando Pandolfo and Jung, Markus and Schulze, Martin
Journal: Animal reproduction science (2022): 107076
Authors: Quirino, Monike and Jakop, Ulrike and Mellagi, Ana Paula Gonçalves and Bortolozzo, Fernando Pandolfo and Jung, Markus and Schulze, Martin
Journal: Animal reproduction science (2022): 107076
Determination of FRET orientation factor between artificial fluorophore and photosynthetic light-harvesting 2 complex (LH2).
Authors: Fujimoto, Kazuhiro J and Miyashita, Tomoya and Dewa, Takehisa and Yanai, Takeshi
Journal: Scientific reports (2022): 15091
Authors: Fujimoto, Kazuhiro J and Miyashita, Tomoya and Dewa, Takehisa and Yanai, Takeshi
Journal: Scientific reports (2022): 15091