XFD647 amine
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1129.14 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Direct upgrades
| iFluor® 647 amine |
Alternative formats
| XFD647 acid |
| XFD647 Azide |
| XFD647 Alkyne |
Related products
| Overview |  SDSProtocol |
Upgrade: iFluor® 647 amine
Molecular weight 1129.14 | Correction Factor (280 nm) 0.03 | Correction Factor (565 nm) 0.1109 | Extinction coefficient (cm -1 M -1) 270000 | Excitation (nm) 650 | Emission (nm) 671 | Quantum yield 0.331 |
XFD647 amine is the same molecule to the Alexa Fluor® 647 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy5 dye. XFD647 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD647 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 88.563 µL | 442.815 µL | 885.63 µL | 4.428 mL | 8.856 mL |
| 5 mM | 17.713 µL | 88.563 µL | 177.126 µL | 885.63 µL | 1.771 mL |
| 10 mM | 8.856 µL | 44.281 µL | 88.563 µL | 442.815 µL | 885.63 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (565 nm) | 0.1109 |
| Extinction coefficient (cm -1 M -1) | 270000 |
| Excitation (nm) | 650 |
| Emission (nm) | 671 |
| Quantum yield | 0.331 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 amine *Same Structure to Alexa Fluor™ 488 amine* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD594 amine *Same Structure to Alexa Fluor™ 594 amine* | 375 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| XFD647 maleimide *C2, XFD647 Same Structure to Alexa Fluor™ 647* | 604 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
| XFD532 amine | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD555 amine | 553 | 568 | 155000 | 0.11 | 0.08 | 0.08 |
| XFD568 amine | 579 | 603 | 88000 | 0.691 | 0.45 | 0.46 |
| XFD750 amine | 752 | 776 | 290000 | 0.121 | - | 0.04 |
| ICG amine | 789 | 814 | - | - | - | 0.076 |
Images
Figure 1. XFD647 amine is the same molecule to the Alexa Fluor® 647 amine. It is a cyanine dye with bright red-fluorescence optimal for labeling antibodies.
References
View all 50 references: Citation Explorer
Flow Cytometric Identification and Enumeration of Monocyte Subsets in Bovine and Water Buffalo Peripheral Blood.
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Authors: Grandoni, Francesco and Fraboni, Daniela and Canonico, Barbara and Papa, Stefano and Buccisano, Francesco and Schuberth, Hans-Joachim and Hussen, Jamal
Journal: Current protocols (2023): e676
Diyne inactivators and activity-based fluorescent labeling of phenol hydroxylase in Pseudomonas sp. CF600.
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Authors: Oyarzun Mejia, Alejandra P and Hyman, Michael R
Journal: FEMS microbiology letters (2023)
Cranial Bone Transport Promotes Angiogenesis, Neurogenesis, and Modulates Meningeal Lymphatic Function in Middle Cerebral Artery Occlusion Rats.
Authors: Bai, Shanshan and Lu, Xuan and Pan, Qi and Wang, Bin and Pong U, Kin and Yang, Yongkang and Wang, Haixing and Lin, Sien and Feng, Lu and Wang, Yan and Li, Yucong and Lin, Weiping and Wang, Yujia and Zhang, Xiaoting and Li, Yuan and Li, Linlong and Yang, Zhengmeng and Wang, Ming and Lee, Wayne Yuk-Wai and Jiang, Xiaohua and Li, Gang
Journal: Stroke (2022): 1373-1385
Authors: Bai, Shanshan and Lu, Xuan and Pan, Qi and Wang, Bin and Pong U, Kin and Yang, Yongkang and Wang, Haixing and Lin, Sien and Feng, Lu and Wang, Yan and Li, Yucong and Lin, Weiping and Wang, Yujia and Zhang, Xiaoting and Li, Yuan and Li, Linlong and Yang, Zhengmeng and Wang, Ming and Lee, Wayne Yuk-Wai and Jiang, Xiaohua and Li, Gang
Journal: Stroke (2022): 1373-1385
Mapping the Nicotinic Acetylcholine Receptor Nanocluster Topography at the Cell Membrane with STED and STORM Nanoscopies.
Authors: Saavedra, Lucas A and Buena-Maizón, Héctor and Barrantes, Francisco J
Journal: International journal of molecular sciences (2022)
Authors: Saavedra, Lucas A and Buena-Maizón, Héctor and Barrantes, Francisco J
Journal: International journal of molecular sciences (2022)
Cell-based flow cytometry assay for simultaneous detection of multiple autoantibodies in a single serum sample.
Authors: Tanimura, Yukihiro and Hiroaki, Yoko and Mori, Masahiro and Fujiyoshi, Yoshinori
Journal: Analytical biochemistry (2022): 114721
Authors: Tanimura, Yukihiro and Hiroaki, Yoko and Mori, Masahiro and Fujiyoshi, Yoshinori
Journal: Analytical biochemistry (2022): 114721
Determination of association equilibrium constant from single molecule fluorescence localization microscopy.
Authors: Cappellari, M Victoria and Marcano-García, Luis F and Simoncelli, Sabrina and Aramendía, Pedro F
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 1751-1760
Authors: Cappellari, M Victoria and Marcano-García, Luis F and Simoncelli, Sabrina and Aramendía, Pedro F
Journal: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society (2022): 1751-1760
3D Single Molecule Super-Resolution Microscopy of Whole Nuclear Lamina.
Authors: Rozario, Ashley M and Morey, Alison and Elliott, Cade and Russ, Brendan and Whelan, Donna R and Turner, Stephen J and Bell, Toby D M
Journal: Frontiers in chemistry (2022): 863610
Authors: Rozario, Ashley M and Morey, Alison and Elliott, Cade and Russ, Brendan and Whelan, Donna R and Turner, Stephen J and Bell, Toby D M
Journal: Frontiers in chemistry (2022): 863610
Organotropic dendrons with high potency as HIV-1, HIV-2 and EV-A71 cell entry inhibitors.
Authors: Martí-Marí, Olaia and Martínez-Gualda, Belén and Fernández-Barahona, Irene and Mills, Alberto and Abdelnabi, Rana and Noppen, Sam and Neyts, Johan and Schols, Dominique and Camarasa, María-José and Herranz, Fernando and Gago, Federico and San-Félix, Ana
Journal: European journal of medicinal chemistry (2022): 114414
Authors: Martí-Marí, Olaia and Martínez-Gualda, Belén and Fernández-Barahona, Irene and Mills, Alberto and Abdelnabi, Rana and Noppen, Sam and Neyts, Johan and Schols, Dominique and Camarasa, María-José and Herranz, Fernando and Gago, Federico and San-Félix, Ana
Journal: European journal of medicinal chemistry (2022): 114414
Development of novel reagents to chicken FLT3, XCR1 and CSF2R for the identification and characterization of avian conventional dendritic cells.
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Authors: Wu, Zhiguang and Hu, Tuanjun and Chintoan-Uta, Cosmin and Macdonald, Joni and Stevens, Mark P and Sang, Helen and Hume, David A and Kaiser, Pete and Balic, Adam
Journal: Immunology (2022): 171-194
Bivalent EGFR-Targeting DARPin-MMAE Conjugates.
Authors: Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M
Journal: International journal of molecular sciences (2022)
Authors: Karsten, Lennard and Janson, Nils and Le Joncour, Vadim and Alam, Sarfaraz and Müller, Benjamin and Tanjore Ramanathan, Jayendrakishore and Laakkonen, Pirjo and Sewald, Norbert and Müller, Kristian M
Journal: International journal of molecular sciences (2022)