Name: XFD647 C2 Maleimide
Brand: AAT Bioquest
SKU: 1895
Price: 237 USD
Availability: InStock

XFD647 C2 Maleimide

XFD647 C2 maleimide is a thiol-reactive, far-red fluorescent dye with an excitation/emission of 650/671 nm that is used for flow cytometry and microscopy applications.

Versatile Conjugation: Efficiently labels thiol groups on proteins, antibodies, and oligonucleotides with stable thioether bonds
Bright & Stable: Delivers intense fluorescence with resilience to photobleaching and pH variations from 4 to 10
Hydrophilic: Minimizes aggregation, enhancing signal clarity for advanced imaging and live-cell studies

Schematic illustrating the maleimide–thiol reaction between a maleimide‐functionalized dye and a thiol‐modified biomolecule. This method is widely used for site‐specific conjugation to cysteine residues in proteins or to thiolated oligonucleotides. Maleimide–thiol coupling proceeds efficiently at or near physiological pH and results in a stable thioether bond.

Protocol

SDS

Catalog	Size	Price	Quantity
1895	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD647 C2 Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	91.312 µL	456.558 µL	913.117 µL	4.566 mL	9.131 mL
5 mM	18.262 µL	91.312 µL	182.623 µL	913.117 µL	1.826 mL
10 mM	9.131 µL	45.656 µL	91.312 µL	456.558 µL	913.117 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1095.15 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments

Authors:

Patil, M. K., Kotresh, M. G., Inamdar, S. R.

Journal:

Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line

Authors:

Ostad, S. N., Babaei, S., Bayat, A. A., Mahmoudian, J.

Journal:

Monoclon Antib Immunodiagn Immunother (2019): 25-29

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry

Authors:

Rai, S., Bhardwaj, U., Misra, A., Singh, S., Gupta, R.

Journal:

Int J Lab Hematol (2018): e52-e54

Development of new hCaM-Alexa Fluor((R)) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I., Leon-Cruz, E., Pardo, J. P., Sosa-Peinado, A., Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui, J. J., Zhu, X. L., Ji, C. F., Jing, X. H., Bai, W. Z.

Journal:

Zhen Ci Yan Jiu (2011): 262-7

References

View all 39 references: Citation Explorer

Development of new hCaM-Alexa Fluor(R) biosensors for a wide range of ligands

Authors:

Velazquez-Lopez, I.; Leon-Cruz, E.; Pardo, J. P.; Sosa-Peinado, A.; Gonzalez-Andrade, M.

Journal:

Anal Biochem (2017): 13-22

Synthetic Protocol for AFCS: A Biologically Active Fluorescent Castasterone Analog Conjugated to an Alexa Fluor 647 Dye

Authors:

Winne, J. M.; Irani, N. G.; Van den Begin, J.; Madder, A.

Journal:

Methods Mol Biol (2017): 21-Sep

Alteration of AMPA Receptor-Mediated Synaptic Transmission by Alexa Fluor 488 and 594 in Cerebellar Stellate Cells

Authors:

Maroteaux, M.; Liu, S. J.

Journal:

eNeuro (2016)

Alexa fluor-labeled fluorescent cellulose nanocrystals for bioimaging solid cellulose in spatially structured microenvironments

Authors:

Grate, J. W.; Mo, K. F.; Shin, Y.; Vasdekis, A.; Warner, M. G.; Kelly, R. T.; Orr, G.; Hu, D.; Dehoff, K. J.; Brockman, F. J.; Wilkins, M. J.

Journal:

Bioconjug Chem (2015): 593-601

In vivo visualization of GL261-luc2 mouse glioma cells by use of Alexa Fluor-labeled TRP-2 antibodies

Authors:

Fenton, K. E.; Martirosyan, N. L.; Abdelwahab, M. G.; Coons, S. W.; Preul, M. C.; Scheck, A. C.

Journal:

Neurosurg Focus (2014): E12

Physical properties

Molecular weight	1095.15
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.00
Correction factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33 ¹

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on April 4, 2026