Name: XFD647-Wheat Germ Agglutinin (WGA) Conjugate
Brand: AAT Bioquest
SKU: 25512
Price: 111 USD
Availability: InStock

XFD647-Wheat Germ Agglutinin (WGA) Conjugate

XFD647, manufactured by AAT Bioquest, has a similar chemical structure to Alexa Fluor® 647, a trademark of Thermo Fisher. Wheat germ agglutinin (WGA) is a well-researched lectin known for its valuable biological applications. Due to its ability to bind to glycoconjugates, WGA derivatives and conjugates are widely used to label yeast bud scars, fibrotic scar tissue, and the cell membrane of gram bacteria and mammalian cells. WGA specifically targets sequences of β-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer possesses two identical, non-interacting binding sites that complement 3 or 4 β-1,4-GlcNAc units. Among the tested monosaccharides, only GlcNAc exhibits binding to WGA, while ManNAc does not bind, and GalNAc demonstrates weak binding. XFD647 WGA conjugate, like its counterpart Alexa Fluor® 647 WGA conjugate, emits a bright red fluorescence and is useful in a variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), and flow cytometry (FC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
25512	1 mg	Price

References

View all 10 references: Citation Explorer

Chitin distribution in the Oithona digestive and reproductive systems revealed by fluorescence microscopy.

Authors:

Sugier, Kevin and Vacherie, Benoit and Cornils, Astrid and Wincker, Patrick and Jamet, Jean-Louis and Madoui, Mohammed-Amin

Journal:

PeerJ (2018): e4685

Chairside molecular imaging of aberrant glycosylation in subjects with suspicious oral lesions using fluorescently labeled wheat germ agglutinin.

Authors:

Baeten, John and Johnson, Alexander and Sunny, Sumsum and Suresh, Amritha and Birur, Praveen and Uma, K and Kademani, Deepak

Journal:

Head & neck (2018): 292-301

Antifungal curcumin promotes chitin accumulation associated with decreased virulence of Sporothrix schenckii.

Authors:

Huang, Lilin and Zhang, Jing and Song, Tianzhang and Yuan, Liyan and Zhou, Junjie and Yin, Hongling and He, Tailong and Gao, Wenchao and Sun, Yao and Hu, Xuchu and Huang, Huaiqiu

Journal:

International immunopharmacology (2016): 263-270

Endothelial glycocalyx structure in the intact carotid artery: a two-photon laser scanning microscopy study.

Authors:

Reitsma, Sietze and oude Egbrink, Mirjam G A and Vink, Hans and van den Berg, Bernard M and Passos, Valéria Lima and Engels, Wim and Slaaf, Dick W and van Zandvoort, Marc A M J

Journal:

Journal of vascular research (2011): 297-306

Isolation and characterization of Saccharomyces cerevisiae mutants resistant to Calcofluor white.

Authors:

Roncero, C and Valdivieso, M H and Ribas, J C and Durán, A

Journal:

Journal of bacteriology (1988): 1950-4

Physical properties

Solvent

Water

Spectral properties

Correction factor (260 nm)	0.00
Correction factor (280 nm)	0.03
Extinction coefficient (cm ^-1 M ^-1)	239000
Excitation (nm)	650
Emission (nm)	671
Quantum yield	0.33 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom

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Page updated on April 30, 2026