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XFD750 amine

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Physical properties
Molecular weight1155.17
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.04
Correction Factor (565 nm)0.0277
Correction Factor (650 nm)0.1505
Extinction coefficient (cm -1 M -1)290000
Excitation (nm)752
Emission (nm)776
Quantum yield0.121
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Direct upgrades
iFluor® 750 amine
Alternative formats
XFD750 acid
Related products
XFD488 azide *Same Structure to Alexa Fluor™ 488 azide*
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne*
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*
XFD488 Hydroxylamine *Same Structure to Alexa Fluor™ 488 Hydroxylamine*
XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*
XFD350-streptavidin conjugate *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488-streptavidin conjugate *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594-streptavidin conjugate *XFD594 Same Structure to Alexa Fluor™ 594*
XFD350 Phalloidin *XFD350 Same Structure to Alexa Fluor™ 350*
XFD488 Phalloidin *XFD488 Same Structure to Alexa Fluor™ 488*
XFD594 Phalloidin *XFD594 Same Structure to Alexa Fluor™ 594*
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*
XFD488 tyramide reagent *Same Structure to Alexa Fluor™ 488 tyramide*
XFD546 tyramide reagent *Same Structure to Alexa Fluor™ 546 tyramide*
XFD594 tyramide reagent *Same Structure to Alexa Fluor™ 594 tyramide*
XFD488 NHS Ester-UltraPure Grade *XFD488 Same Structure to Alexa Fluor™ 488*
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*
XFD647 C2 Maleimide *Same Structure to Alexa Fluor™ 647 C2 Maleimide*
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*
XFD350 tyramide reagent *Same Structure to Alexa Fluor™ 350 tyramide*
XFD568 tyramide reagent *Same Structure to Alexa Fluor™ 568 tyramide*
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*
XFD488 aldehyde *Same Structure to Alexa Fluor™ 488 aldehyde*
XFD™488-dUTP *1 mM in Tris Buffer (pH 7.5)*
XFD514 acid
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*
XFD514 tyramide
XFD532 tyramide
XFD647 Azide
XFD647 Alkyne
XFD488 TCO
XFD555 acid
XFD647 acid
XFD700 acid
XFD647 Phalloidin *equivalent to Alexa Fluor® 647 phalloidin*
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]
Show More (47)

OverviewpdfSDSpdfProtocol


Molecular weight
1155.17
Correction Factor (280 nm)
0.04
Correction Factor (565 nm)
0.0277
Correction Factor (650 nm)
0.1505
Extinction coefficient (cm -1 M -1)
290000
Excitation (nm)
752
Emission (nm)
776
Quantum yield
0.121
XFD750 amine is the same molecule to the Alexa Fluor® 750 amine (Alexa Fluor® is the trademark of ThermoFisher). It is a cyanine dye with bright NIR-fluorescence optimal for labeling antibodies. It has the identical spectra to Cy7 dye. XFD750 dye is water soluble and pH-insensitive from pH 4 to 10. The amine of XFD750 is a versatile carbonyl-reactive form for conjugating this fluorophore to peptides and other biological molecules.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD750 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.567 µL432.837 µL865.673 µL4.328 mL8.657 mL
5 mM17.313 µL86.567 µL173.135 µL865.673 µL1.731 mL
10 mM8.657 µL43.284 µL86.567 µL432.837 µL865.673 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.04
Correction Factor (565 nm)0.0277
Correction Factor (650 nm)0.1505
Extinction coefficient (cm -1 M -1)290000
Excitation (nm)752
Emission (nm)776
Quantum yield0.121

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.300.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.430.56
XFD532 amine534553810000.6110.240.09
XFD555 amine5535681550000.110.080.08
XFD568 amine579603880000.6910.450.46
XFD647 amine6506712700000.331-0.03
ICG amine789814---0.076

Images


References


View all 31 references: Citation Explorer
pH-responsive graphene oxide loaded with targeted peptide and anticancer drug for OSCC therapy.
Authors: Li, Ran and Gao, Ruifang and Zhao, Yingjiao and Zhang, Fang and Wang, Xiangyu and Li, Bing and Wang, Lu and Ma, Lixin and Du, Jie
Journal: Frontiers in oncology (2022): 930920
Near-Infrared Fluorescence Imaging of Carotid Plaques in an Atherosclerotic Murine Model.
Authors: Wu, Xiaotian and Daniel Ulumben, Amy and Long, Steven and Katagiri, Wataru and Wilks, Moses Q and Yuan, Hushan and Cortese, Brian and Yang, Chengeng and Kashiwagi, Satoshi and Choi, Hak Soo and Normandin, Marc D and El Fakhri, Georges and Zaman, Raiyan T
Journal: Biomolecules (2021)
Challenging a Preconception: Optoacoustic Spectrum Differs from the Optical Absorption Spectrum of Proteins and Dyes for Molecular Imaging.
Authors: Fuenzalida Werner, Juan Pablo and Huang, Yuanhui and Mishra, Kanuj and Janowski, Robert and Vetschera, Paul and Heichler, Christina and Chmyrov, Andriy and Neufert, Clemens and Niessing, Dierk and Ntziachristos, Vasilis and Stiel, Andre C
Journal: Analytical chemistry (2020)
CD24-targeted intraoperative fluorescence image-guided surgery leads to improved cytoreduction of ovarian cancer in a preclinical orthotopic surgical model.
Authors: Kleinmanns, Katrin and Fosse, Vibeke and Davidson, Ben and de Jalón, Elvira García and Tenstad, Olav and Bjørge, Line and McCormack, Emmet
Journal: EBioMedicine (2020): 102783
Mechanistic profiling of the release kinetics of siRNA from lipidoid-polymer hybrid nanoparticles in vitro and in vivo after pulmonary administration.
Authors: Thanki, Kaushik and van Eetvelde, Delphine and Geyer, Antonia and Fraire, Juan and Hendrix, Remi and Van Eygen, Hannelore and Putteman, Emma and Sami, Haider and de Souza Carvalho-Wodarz, Cristiane and Franzyk, Henrik and Nielsen, Hanne Mørck and Braeckmans, Kevin and Lehr, Claus-Michael and Ogris, Manfred and Foged, Camilla
Journal: Journal of controlled release : official journal of the Controlled Release Society (2019): 82-93
Generation and characterization of novel recombinant anti-hERG1 scFv antibodies for cancer molecular imaging.
Authors: Duranti, Claudia and Carraresi, Laura and Sette, Angelica and Stefanini, Matteo and Lottini, Tiziano and Crescioli, Silvia and Crociani, Olivia and Iamele, Luisa and De Jonge, Hugo and Gherardi, Ermanno and Arcangeli, Annarosa
Journal: Oncotarget (2018): 34972-34989
Enhanced Release of Molecules upon Ultraviolet (UV) Light Irradiation from Photoresponsive Hydrogels Prepared from Bifunctional Azobenzene and Four-Arm Poly(ethylene glycol).
Authors: Rastogi, Shiva K and Anderson, Hailee E and Lamas, Joseph and Barret, Scott and Cantu, Travis and Zauscher, Stefan and Brittain, William J and Betancourt, Tania
Journal: ACS applied materials & interfaces (2018): 30071-30080
Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging.
Authors: Dong, Lili and Zhou, Hongxin and Zhao, Menglong and Gao, Xinghui and Liu, Yang and Liu, Dongli and Guo, Wei and Hu, Hongwei and Xie, Qian and Fan, Jia and Lin, Jiang and Wu, Weizhong
Journal: Molecular therapy. Nucleic acids (2018): 376-386
In vivo fluorescence imaging of hepatocellular carcinoma using a novel GPC3-specific aptamer probe.
Authors: Zhao, Menglong and Dong, Lili and Liu, Zhuang and Yang, Shuohui and Wu, Weizhong and Lin, Jiang
Journal: Quantitative imaging in medicine and surgery (2018): 151-160
Performance of optoacoustic and fluorescence imaging in detecting deep-seated fluorescent agents.
Authors: Chen, Zhenyue and Deán-Ben, Xosé Luís and Gottschalk, Sven and Razansky, Daniel
Journal: Biomedical optics express (2018): 2229-2239