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Xite™ Green beta-D-galactopyranoside
Xite™ Green beta-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase (β-gal). Compared to the existing beta-galactosidase substrates (e.g., the commonly used FDG), it has much better cell permeability. Xite™ Green beta-D-galactopyranoside readily enters cells where it gets cleaved by β-gal, producing Xite™ Green, a strongly fluorescent product. The strongly fluorescent Xite™ Green is well retained in cells, making it easy to be detected with a flow cytometer and fluorescence microscope. Xite™ Green beta-D-galactopyranoside provides a simple and sensitive tool to detect beta-galactosidase activity. Xite™ Green beta-D-galactopyranoside might be used as a simple tool for measuring cellular senescence in cells since β-gal has been identified as a reliable marker for cellular senescence.
Expression of β-gal was measured with Xite™ Green beta-D-galactopyranoside. 9L-LacZ cells (cells that overexpressed β-gal) were incubated with Xite™ Green beta-D-galactopyranoside or FDG for 30 mins at 37 °C. The signal was acquired with FITC channel using a NovoCyte Flow Cytometer (ACEA Biosciences).
Expression of β-gal was measured with Xite™ Green beta-D-galactopyranoside. 9L-LacZ cells (cells that overexpressed β-gal) were incubated with Xite™ Green beta-D-galactopyranoside or FDG for 30 mins at 37 °C. The signal was acquired with FITC channel using a NovoCyte Flow Cytometer (ACEA Biosciences).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
140301 mg
Price
 
Physical properties
Molecular weight494.50
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 30, 2025