Name: XTT Assay
Brand: AAT Bioquest
SKU: 22774
Availability: InStock

XTT Assay

XTT Assay is a ready-to-use cell viability kit that measures metabolic activity through soluble formazan production, ideal for high-throughput proliferation and cytotoxicity screening.

Ready-To-Use Format: Convenient one-bottle, ready-to-use solution that simplifies assay preparation
High-Throughput Capability: Ideal for evaluating cell proliferation and cytotoxicity in large sample sets, supporting efficient screening and analysis
No Washing or Solubilization required: Unlike traditional assays, this assay eliminates the need for washing or solubilization, offering a quicker, easier, and more efficient method

Cell number was determined with XTT Assay. HeLa cells at 1000 to 20,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 450 nm and 660 nm at 4 hours after XTT solution incubation with a ClarioStar absorbance microplate reader. The specific absorbance signals were determined using the equation shown on the protocol and signal/background ratio (well with no cells) was measured and plotted.

Protocol

COA

Catalog	Size	Price	Quantity
22774	1000 Tests	Price
22775	5000 Tests	Price

Overview

The XTT assay is a fast and reliable method for measuring cell viability and proliferation. Based on the reduction of the XTT tetrazolium compound by metabolically active cells, this kit produces a soluble formazan product that can be quantified through absorbance at 450 nm. The assay eliminates the need for washing or solubilization, offering a streamlined workflow and reducing hands-on time.

This XTT assay kit comes in a ready-to-use format making it ideal for high-throughput screening, making it perfect for evaluating cell proliferation in response to various stimuli, such as growth factors, cytokines, and mitogens, as well as assessing cytotoxicity for compounds like anticancer drugs and pharmaceuticals. With its ready-to-use format, this XTT assay is designed to deliver consistent, reproducible results for researchers and labs involved in cell-based assays.

References

View all 50 references: Citation Explorer

XTT assay for detection of bacterial metabolic activity in water-based polyester polyurethane.

Authors:

Magaña-Montiel, Nallely and Muriel-Millán, Luis Felipe and Pardo-López, Liliana

Journal:

PloS one (2024): e0303210

The performance and costs of XTT, resazurin, MTS and luciferin as viability dyes in in vitro susceptibility testing of Madurella mycetomatis.

Authors:

Ma, Jingyi and Eadie, Kimberly and Fahal, Ahmed and Verbon, Annelies and van de Sande, Wendy W J

Journal:

Transactions of the Royal Society of Tropical Medicine and Hygiene (2024): 729-735

Quantitation of novel pentavalent meningococcal polysaccharide conjugate vaccine (Men A-TT, Men C-CRM, Men Y-CRM, Men W-CRM, Men X-TT) using sandwich ELISA.

Authors:

Phugare, Swapnil and Kale, Sameer and Sharma, Pankaj and Kumar Goel, Sunil and Gairola, Sunil and Jadhav, Suresh S

Journal:

Vaccine (2020): 7815-7824

ATP-based cell viability assay is superior to trypan blue exclusion and XTT assay in measuring cytotoxicity of anticancer drugs Taxol and Imatinib, and proteasome inhibitor MG-132 on human hepatoma cell line HepG2.

Authors:

Nowak, Elisabeth and Kammerer, Sarah and Küpper, Jan-Heiner

Journal:

Clinical hemorheology and microcirculation (2018): 327-336

Applying XTT, WST-1, and WST-8 to human chondrocytes: A comparison of membrane-impermeable tetrazolium salts in 2D and 3D cultures.

Authors:

Lutter, Anne-Helen and Scholka, Jenny and Richter, Heiko and Anderer, Ursula

Journal:

Clinical hemorheology and microcirculation (2017): 327-342

Physical properties

Solvent

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C)

Instrument settings

Absorbance microplate reader
Absorbance	450 nm and 660 nm
Recommended plate	Clear bottom

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Page updated on July 15, 2026