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XTT Assay
XTT Assay is a ready-to-use cell viability kit that measures metabolic activity through soluble formazan production, ideal for high-throughput proliferation and cytotoxicity screening.
  • Ready-To-Use Format: Convenient one-bottle, ready-to-use solution that simplifies assay preparation
  • High-Throughput Capability: Ideal for evaluating cell proliferation and cytotoxicity in large sample sets, supporting efficient screening and analysis
  • No Washing or Solubilization required: Unlike traditional assays, this assay eliminates the need for washing or solubilization, offering a quicker, easier, and more efficient method
Cell number was determined with XTT Assay. HeLa cells at 1000 to 20,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 450 nm and 660 nm at 4 hours after XTT solution incubation with a ClarioStar absorbance microplate reader. The specific absorbance signals were determined using the equation shown on the protocol and signal/background ratio (well with no cells) was measured and plotted.
Cell number was determined with XTT Assay. HeLa cells at 1000 to 20,000 cells/well/100 µL were added in a clear bottom 96-well plate. The absorbance was measured at 450 nm and 660 nm at 4 hours after XTT solution incubation with a ClarioStar absorbance microplate reader. The specific absorbance signals were determined using the equation shown on the protocol and signal/background ratio (well with no cells) was measured and plotted.
CatalogSize
Price
Quantity
227741000 Tests
Price
227755000 Tests
Price
 
Physical properties

Solvent
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
Instrument settings

Absorbance microplate reader
Absorbance 450 nm and 660 nm
Recommended plateClear bottom
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Page updated on October 19, 2025