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Z-ATAD-aminoluciferin
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 771.82 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 18000 |
| Excitation (nm) | 362 |
| Emission (nm) | 499 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 771.82 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
Z-ATAD-aminoluciferin is a luminogenic substrate that measures caspase-12 activity in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence ATAD in this substrate is specific for protease cleavage by caspase-12. Following caspase-12 activation, the ATAD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-12 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Z-ATAD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 129.564 µL | 647.819 µL | 1.296 mL | 6.478 mL | 12.956 mL |
| 5 mM | 25.913 µL | 129.564 µL | 259.128 µL | 1.296 mL | 2.591 mL |
| 10 mM | 12.956 µL | 64.782 µL | 129.564 µL | 647.819 µL | 1.296 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 18000 |
| Excitation (nm) | 362 |
| Emission (nm) | 499 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-LETD-aminoluciferin | 362 | 499 | 18000 |
| Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
Images
Figure 1. Chemical structure for Z-ATAD-aminoluciferin.