Z-DEVD-AFC

Z-DEVD-AFC is a fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. This substrate is hydrolyzed by caspase 3 to generate highly fluorescent 7-amido-4-trifluoromethylcoumarin (AFC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13420	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-DEVD-AFC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.697 µL	608.487 µL	1.217 mL	6.085 mL	12.17 mL
5 mM	24.339 µL	121.697 µL	243.395 µL	1.217 mL	2.434 mL
10 mM	12.17 µL	60.849 µL	121.697 µL	608.487 µL	1.217 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	821.71 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Palmitoylation is required for TNF-R1 signaling

Authors:

Zingler, Philipp and Särchen, Vinzenz and Glatter, Timo and Caning, Lotta and Saggau, Carina and Kathayat, Rahul S and Dickinson, Bryan C and Adam, Dieter and Schneider-Brachert, Wulf and Schütze, Stefan and others, undefined

Journal:

Cell Communication and Signaling (2019): 1--16

pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity

Authors:

Yu, Shaoxuan and Wang, Yanru and Zhang, Wentao and Zhang, Yuhuan and Zhu, Wenxin and Liu, Yingnan and Zhang, Daohong and Wang, Jianlong

Journal:

RSC Advances (2016): 72213--72223

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	821.71
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000 ¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025