Z-DEVD-AMC

Z-DEVD-AMC is a fluorogenic substrate for caspase 3, a protease that is rapidly activated when cells are exposed to apoptotic conditions and that cleaves poly(ADP-ribose) polymerase. This substrate is hydrolyzed by caspase 3 to generate highly fluorescent 7-amido-4-methylcoumarin (AMC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13421	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-DEVD-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	130.263 µL	651.313 µL	1.303 mL	6.513 mL	13.026 mL
5 mM	26.053 µL	130.263 µL	260.525 µL	1.303 mL	2.605 mL
10 mM	13.026 µL	65.131 µL	130.263 µL	651.313 µL	1.303 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	767.68 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Herbo-vitamin medicine Livogrit Vital ameliorates isoniazid induced liver injury (IILI) in human liver (HepG2) cells by decreasing isoniazid accumulation and oxidative stress driven hepatotoxicity

Authors:

Balkrishna, Acharya and Gohel, Vivek and Tomer, Meenu and Dev, Rishabh and Varshney, Anurag

Journal:

BMC Complementary Medicine and Therapies (2024): 386

Effect of Statin Lipophilicity on the Proliferation of Hepatocellular Carcinoma Cells

Authors:

Glebavi{\v{c}}i{\=u}t{\.e}, Goda and Vijaya, Akshay Kumar and Preta, Giulio

Journal:

Biology (2024): 455

pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity

Authors:

Yu, Shaoxuan and Wang, Yanru and Zhang, Wentao and Zhang, Yuhuan and Zhu, Wenxin and Liu, Yingnan and Zhang, Daohong and Wang, Jianlong

Journal:

RSC Advances (2016): 72213--72223

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	767.68
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 22, 2025