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Z-DEVD-aminoluciferin
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 871.89 |
| Solvent | DMSO |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 18000 |
| Excitation (nm) | 362 |
| Emission (nm) | 499 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 871.89 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
Z-DEVD-aminoluciferin is a luminogenic substrate that measures caspase-3 and -7 activities in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. Notably, caspases-3 and -7, target the DEVD motif in poly(ADP-ribose) polymerase (PARP), disrupting the repair capabilities of PARP and facilitating DNA fragmentation, a hallmark of apoptosis. When caspase-3/7 enzymes cleave the Z-DEVD-aminoluciferin substrate, it releases aminoluciferin, which can react with luciferase to produce bioluminescence. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-3/7 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival. Z-DEVD-aminoluciferin is a modified version of the Ac-DEVD-aminoluciferin substrate in that it employs Z (carbobenzoxy or benzyloxycarbonyl) as the protective group in place of acetyl.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Z-DEVD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 114.693 µL | 573.467 µL | 1.147 mL | 5.735 mL | 11.469 mL |
| 5 mM | 22.939 µL | 114.693 µL | 229.387 µL | 1.147 mL | 2.294 mL |
| 10 mM | 11.469 µL | 57.347 µL | 114.693 µL | 573.467 µL | 1.147 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 18000 |
| Excitation (nm) | 362 |
| Emission (nm) | 499 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Z-DEVD-AFC | 376 | 482 | 170001 |
| Z-DEVD-AMC | 341 | 441 | - |
| Ac-DEVD-aminoluciferin | 362 | 499 | 18000 |
| Z-IETD-aminoluciferin | 362 | 499 | 18000 |
| Z-LETD-aminoluciferin | 362 | 499 | 18000 |
| Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
| Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
| Z-VEID-aminoluciferin | 362 | 499 | 18000 |
| Z-ATAD-aminoluciferin | 362 | 499 | 18000 |
Images
Figure 1. Chemical structure for Z-DEVD-aminoluciferin.