Z-DEVD-ProRed™ 620

ProRed™-derived protease substrates are colorless and non-fluorescent. Cleavage of blocking protease-cleavable peptide residue by caspases generates the strongly red fluorescent ProRed™ that can be monitored fluorimetrically at ~620 nm with excitation of ~530 nm. ProRed™-derived caspase substrates are the most sensitive red indicators for the fluorimetric detection of various caspase activities. This DEVD-ProRed™ substrate is specific for detecting caspases 3 and 7.

Detection of Caspase 3/7 Activities in Jurkat cells. <br />Jurkat cells were seeded on the same day at 200,000 cells/90 uL/well in a Costar black wall/clear bottom 96-well plate. The cells were treated with staurosporine at the final concentration of 1 uM for 5 hours while the untreated cells were used as control. The Z-DEVD-ProRed™ 620 assay solution (100 uL/well) was added and incubated at room temperature for 1 hour. The fluorescence intensity was measured at Ex/Em = 540/620 nm with FlexStation fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13433	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-DEVD-ProRed™ 620 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	63.877 µL	319.387 µL	638.774 µL	3.194 mL	6.388 mL
5 mM	12.775 µL	63.877 µL	127.755 µL	638.774 µL	1.278 mL
10 mM	6.388 µL	31.939 µL	63.877 µL	319.387 µL	638.774 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1565.5 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Degenerin channel activation causes caspase-mediated protein degradation and mitochondrial dysfunction in adult C. elegans muscle

Authors:

Gaffney, Christopher J and Shephard, Freya and Chu, Jeff and Baillie, David L and Rose, Ann and Constantin-Teodosiu, Dumitru and Greenhaff, Paul L and Szewczyk, Nathaniel J

Journal:

Journal of cachexia, sarcopenia and muscle (2016): 181--192

Degenerin channel activation causes caspase-mediated protein degradation and mitochondrial dysfunction in adult C. elegans muscle

Authors:

Gaffney, Christopher J and Shephard, Freya and Chu, Jeff and Baillie, David L and Rose, Ann and Constantin-Teodosiu, Dumitru and Greenhaff, Paul L and Szewczyk, Nathaniel J

Journal:

Journal of Cachexia, Sarcopenia and Muscle (2015)

References

View all 101 references: Citation Explorer

Fisetin induces apoptosis in human cervical cancer HeLa cells through ERK1/2-mediated activation of caspase-8-/caspase-3-dependent pathway

Authors:

Ying TH, Yang SF, Tsai SJ, Hsieh SC, Huang YC, Bau DT, Hsieh YH.

Journal:

Arch Toxicol (2012): 263

Andrographolide induces apoptosis in B16F-10 melanoma cells by inhibiting NF-kappaB-mediated bcl-2 activation and modulating p53-induced caspase-3 gene expression

Authors:

Pratheeshkumar P, Sheeja K, Kuttan G.

Journal:

Immunopharmacol Immunotoxicol (2012): 143

ECRG4 is a negative regulator of caspase-8-mediated apoptosis in human T-leukemia cells

Authors:

Matsuzaki J, Torigoe T, Hirohashi Y, Kamiguchi K, Tamura Y, Tsukahara T, Kubo T, Takahashi A, Nakazawa E, Saka E, Yasuda K, Takahashi S, Sato N.

Journal:

Carcinogenesis (2012): 996

Proteasome inhibition can impair caspase-8 Activation upon sub-maximal Stimulation of apoptotic tumour necrosis factor-related apoptosis inducing ligand (TRAIL) signalling

Authors:

Laussmann MA, Passante E, Hellwig CT, Tomiczek B, Flanagan L, Prehn JH, Huber HJ, Rehm M.

Journal:

J Biol Chem. (2012)

Caspase-2 is an initiator caspase responsible for pore-forming toxin-mediated apoptosis

Authors:

Imre G, Heering J, Takeda AN, Husmann M, Thiede B, Zu Heringdorf DM, Green DR, van der Goot FG, Sinha B, Dotsch V, Rajalingam K.

Journal:

EMBO J. (2012)

Physical properties

Molecular weight	1565.50
Solvent	DMSO

Spectral properties

Excitation (nm)	532
Emission (nm)	619

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025