Name: Z-IETD-AFC *CAS 219138-02-0*
Brand: AAT Bioquest
SKU: 13425
Price: 148 USD
Availability: InStock

Z-IETD-AFC

CAS 219138-02-0

Z-IETD-AFC is a fluorogenic caspase-8/granzyme B substrate containing the acetyl (Ac) moiety. This substrate is hydrolyzed by caspase 8 to generate highly fluorescent 7-amido-4-trifluoromethylcoumarin (AFC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13425	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-IETD-AFC *CAS 219138-02-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.692 µL	608.458 µL	1.217 mL	6.085 mL	12.169 mL
5 mM	24.338 µL	121.692 µL	243.383 µL	1.217 mL	2.434 mL
10 mM	12.169 µL	60.846 µL	121.692 µL	608.458 µL	1.217 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	821.75 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

ONC206, an imipridone derivative, induces cell death through activation of the integrated stress response in serous endometrial cancer in vitro

Authors:

Zhang, Yingao and Huang, Yu and Yin, Yajie and Fan, Yali and Sun, Wenchuan and Zhao, Xiaoling and Tucker, Katherine and Staley, Allison and Paraghamian, Sarah and Hawkins, Gabrielle and others,

Journal:

Frontiers in Oncology (2020): 577141

pH-Assisted surface functionalization of selenium nanoparticles with curcumin to achieve enhanced cancer chemopreventive activity

Authors:

Yu, Shaoxuan and Wang, Yanru and Zhang, Wentao and Zhang, Yuhuan and Zhu, Wenxin and Liu, Yingnan and Zhang, Daohong and Wang, Jianlong

Journal:

RSC Advances (2016): 72213--72223

References

View all 67 references: Citation Explorer

In vivo and in vitro sensitization of leukemic cells to adriamycin-induced apoptosis by pentoxifylline. Involvement of caspase cascades and IkappaBalpha phosphorylation

Authors:

Lerma-Diaz JM, Hern and ez-Flores G, Dominguez-Rodriguez JR, Ortiz-Lazareno PC, Gomez-Contreras P, Cervantes-Munguia R, Scott-Algara D, Aguilar-Lemarroy A, Jave-Suarez LF, Bravo-Cuellar A.

Journal:

Immunol Lett (2006): 149

Measurement of two caspase activities simultaneously in living cells by a novel dual FRET fluorescent indicator probe

Authors:

Wu X, Simone J, Hewgill D, Siegel R, Lipsky PE, He L.

Journal:

Cytometry A (2006): 477

Quantitative measurement of caspase-3 activity in a living starfish egg

Authors:

Sakaue M, Motoyama Y, Yamamoto K, Shiba T, Teshima T, Chiba K.

Journal:

Biochem Biophys Res Commun (2006): 878

Photoreceptor cell apoptosis induced by the 2-nitroimidazole radiosensitizer, CI-1010, is mediated by p53-linked activation of caspase-3

Authors:

Miller TJ, Schneider RJ, Miller JA, Martin BP, Al-Ubaidi MR, Agarwal N, Dethloff LA, Philbert MA.

Journal:

Neurotoxicology (2006): 44

Diallyl Trisulfide Induces Apoptosis of Human Gastric Cancer Cell Line MGC803 Through Caspase-3 Pathway.

Authors:

Xiao XL, Peng J, Su Q, Xiang SL, Tang GH, Huang YS, Zhou XT.

Journal:

Ai Zheng (2006): 1247

Physical properties

Molecular weight	821.75
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	17000 ¹
Excitation (nm)	376
Emission (nm)	482
Quantum yield	0.53 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	219138-02-0

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 27, 2025