Z-IETD-aminoluciferin
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Additional ordering information
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Physical properties
Molecular weight | 871.93 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Molecular weight 871.93 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
Z-IETD-aminoluciferin is a luminogenic substrate that measures caspase-6 and granzyme B activities in apoptotic cells. Caspases, a family of aspartate-specific cysteine proteases, are key regulators of apoptosis, orchestrating the initiation and execution of cellular disassembly through the cleavage of specific cellular proteins. The tetrapeptide sequence IETD in this substrate is specific for protease cleavage by caspase-6. Following caspase-6 activation, the IETD peptide is cleaved just after the aspartic acid residue (D), releasing aminoluciferin, which serves as a luminescent substrate for the luciferase reaction. The intensity of the bioluminescent signal is directly proportional to the amount of caspase-6 activity in the sample, making it a valuable tool for researchers to study and quantify apoptotic processes, screen potential drug candidates, and investigate various cellular pathways related to cell death and survival.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Z-IETD-aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 114.688 µL | 573.441 µL | 1.147 mL | 5.734 mL | 11.469 mL |
5 mM | 22.938 µL | 114.688 µL | 229.376 µL | 1.147 mL | 2.294 mL |
10 mM | 11.469 µL | 57.344 µL | 114.688 µL | 573.441 µL | 1.147 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Z-IETD-AFC *CAS 219138-02-0* | 376 | 482 | 170001 |
Z-DEVD-aminoluciferin | 362 | 499 | 18000 |
Z-LETD-aminoluciferin | 362 | 499 | 18000 |
Z-LEHD-aminoluciferin | 362 | 499 | 18000 |
Z-VDVAD-aminoluciferin | 362 | 499 | 18000 |
Z-VEID-aminoluciferin | 362 | 499 | 18000 |
Z-ATAD-aminoluciferin | 362 | 499 | 18000 |