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Z-KKAG-AMC

Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was determined as a cause for several cases of respiratory disease (Covid-19). Even though most infected patients only suffer from mild symptoms such as fever and cough associated with a good prognosis, the disease can progress into fatal cases of pneumonia and acute respiratory failure, especially in older males with comorbidities. Covid-19 rapidly spread worldwide. As of May 31st, 2022, more than 6.2 million people have died from coronavirus worldwide, and ~530 million cases have been reported. Coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Like the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), SARS-CoV-2 encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus operates on more than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential, therefore, the main proteases are identified as an attractive drug targets for preventing virus imitation. Papain-like protease (PLpro) of coronaviruses carries out proteolytic maturation of non-structural proteins that play a role in replication of the virus and performs deubiquitination of host cell factors to scuttle antiviral responses. Z-KKAG-AMC is cleaved by papain-like proteases to give the highly fluorescent AMC product. The fluorescence intensity of released AMC is proportional to the protease activity.Z-KKAG-AMC might be used for screening and studying kinetics of PLpro inhibitors.

Spectrum

Product family

NameExcitation (nm)Emission (nm)
Z-DEVD-AMC341441
Z-LRGG-AMC341441
Z-KAGG-AMC341441

References

View all 78 references: Citation Explorer
Measuring digestive protease activation in the mouse pancreas.
Authors: Mosztbacher, Dóra and Demcsák, Alexandra and Sahin-Tóth, Miklós
Journal: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] (2020): 288-292
Discovery of Ubiquitin-Specific Protease 7 (USP7) Inhibitors with Novel Scaffold Structures by Virtual Screening, Molecular Dynamics Simulation, and Biological Evaluation.
Authors: Liu, Shengjie and Zhou, Xinyu and Li, Minglei and Zhao, Wenfeng and Zhou, Shuxi and Cheng, Keguang and Xu, Qinglong and Chen, Caiping and Wen, Xiaoan and Sun, Hongbin and Yuan, Haoliang
Journal: Journal of chemical information and modeling (2020)
Protease-Activated Receptors 2-Antagonist Suppresses Asthma by Inhibiting Reactive Oxygen Species-Thymic Stromal Lymphopoietin Inflammation and Epithelial Tight Junction Degradation.
Authors: Kim, Ha Jung and Lee, Seung Hwa and Jeong, Sekyoo and Hong, Soo Jong
Journal: Allergy, asthma & immunology research (2019): 560-571
Differential Cleaving of Specific Substrates for Cathepsin-Like Activity Shows Cysteine and Serine Protease Activities and a Differential Profile Between Anisakis simplex s.s. and Anisakis pegreffii, Sibling Species Major Etiologic Agents of Anisakiasis.
Authors: Torralbo-Ramírez, Verónica and Molina-Fernández, Dolores and Malagón, David and Benítez, Rocío and Adroher, Francisco Javier
Journal: Foodborne pathogens and disease (2019): 744-751
Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues.
Authors: Kunz, Kathrin and Müller, Stefan and Mendler, Luca
Journal: Methods in enzymology (2019): 389-410
Page updated on October 4, 2024

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Catalog Number13554
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Physical properties

Molecular weight

693.79

Solvent

DMSO

Spectral properties

Excitation (nm)

341

Emission (nm)

441

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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