AAT Bioquest


Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight693.79
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was determined as a cause for several cases of respiratory disease (Covid-19). Even though most infected patients only suffer from mild symptoms such as fever and cough associated with a good prognosis, the disease can progress into fatal cases of pneumonia and acute respiratory failure, especially in older males with comorbidities. Covid-19 rapidly spread worldwide. As of May 31st, 2022, more than 6.2 million people have died from coronavirus worldwide, and ~530 million cases have been reported. Coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Like the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), SARS-CoV-2 encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus operates on more than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential, therefore, the main proteases are identified as an attractive drug targets for preventing virus imitation. Papain-like protease (PLpro) of coronaviruses carries out proteolytic maturation of non-structural proteins that play a role in replication of the virus and performs deubiquitination of host cell factors to scuttle antiviral responses. Z-KKAG-AMC is cleaved by papain-like proteases to give the highly fluorescent AMC product. The fluorescence intensity of released AMC is proportional to the protease activity.Z-KKAG-AMC might be used for screening and studying kinetics of PLpro inhibitors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-KKAG-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM144.136 µL720.679 µL1.441 mL7.207 mL14.414 mL
5 mM28.827 µL144.136 µL288.272 µL1.441 mL2.883 mL
10 mM14.414 µL72.068 µL144.136 µL720.679 µL1.441 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles


Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)341
Emission (nm)441

Product Family

NameExcitation (nm)Emission (nm)



View all 78 references: Citation Explorer
Measuring digestive protease activation in the mouse pancreas.
Authors: Mosztbacher, Dóra and Demcsák, Alexandra and Sahin-Tóth, Miklós
Journal: Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.] (2020): 288-292
Discovery of Ubiquitin-Specific Protease 7 (USP7) Inhibitors with Novel Scaffold Structures by Virtual Screening, Molecular Dynamics Simulation, and Biological Evaluation.
Authors: Liu, Shengjie and Zhou, Xinyu and Li, Minglei and Zhao, Wenfeng and Zhou, Shuxi and Cheng, Keguang and Xu, Qinglong and Chen, Caiping and Wen, Xiaoan and Sun, Hongbin and Yuan, Haoliang
Journal: Journal of chemical information and modeling (2020)
Use of Contemporary Protease Inhibitors and Risk of Incident Chronic Kidney Disease in Persons With Human Immunodeficiency Virus: the Data Collection on Adverse Events of Anti-HIV Drugs (D:A:D) Study.
Authors: Ryom, Lene and Dilling Lundgren, Jens and Reiss, Peter and Kirk, Ole and Law, Matthew and Ross, Mike and Morlat, Phillip and Andreas Fux, Christoph and Fontas, Eric and De Wit, Stephane and D'Arminio Monforte, Antonella and El-Sadr, Wafaa and Phillips, Andrew and Ingrid Hatleberg, Camilla and Sabin, Caroline and Mocroft, Amanda and ,
Journal: The Journal of infectious diseases (2019): 1629-1634
Identification and characteristics of a cathepsin L-like cysteine protease from Clonorchis sinensis.
Authors: Ma, Changling and Liang, Kai and Tang, Lili and He, Shanshan and Liu, Xiaoquan and He, Mian and Li, Yanwen
Journal: Parasitology research (2019): 829-835
Characterization of a novel cathepsin L-like protease from Taenia solium metacestodes for the immunodiagnosis of porcine cysticercosis.
Authors: León-Janampa, Nancy and Liendo, Ruddy and Gilman, Robert H and Padilla, Carlos and García, Hector H and Gonzales, Armando and Sheen, Patricia and Pajuelo, Mónica J and Zimic, Mirko and ,
Journal: Veterinary parasitology (2019): 9-16
Insights into AMS/PCAT transporters from biochemical and structural characterization of a double Glycine motif protease.
Authors: Bobeica, Silvia C and Dong, Shi-Hui and Huo, Liujie and Mazo, Nuria and McLaughlin, Martin I and Jiménez-Osés, Gonzalo and Nair, Satish K and van der Donk, Wilfred A
Journal: eLife (2019)
Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissues.
Authors: Kunz, Kathrin and Müller, Stefan and Mendler, Luca
Journal: Methods in enzymology (2019): 389-410
Protease-Activated Receptors 2-Antagonist Suppresses Asthma by Inhibiting Reactive Oxygen Species-Thymic Stromal Lymphopoietin Inflammation and Epithelial Tight Junction Degradation.
Authors: Kim, Ha Jung and Lee, Seung Hwa and Jeong, Sekyoo and Hong, Soo Jong
Journal: Allergy, asthma & immunology research (2019): 560-571
Differential Cleaving of Specific Substrates for Cathepsin-Like Activity Shows Cysteine and Serine Protease Activities and a Differential Profile Between Anisakis simplex s.s. and Anisakis pegreffii, Sibling Species Major Etiologic Agents of Anisakiasis.
Authors: Torralbo-Ramírez, Verónica and Molina-Fernández, Dolores and Malagón, David and Benítez, Rocío and Adroher, Francisco Javier
Journal: Foodborne pathogens and disease (2019): 744-751
Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure.
Authors: Akkerdaas, Jaap and Totis, Muriel and Barnett, Brian and Bell, Erin and Davis, Tom and Edrington, Thomas and Glenn, Kevin and Graser, Gerson and Herman, Rod and Knulst, Andre and Ladics, Gregory and McClain, Scott and Poulsen, Lars K and Ranjan, Rakesh and Rascle, Jean-Baptiste and Serrano, Hector and Speijer, Dave and Wang, Rong and Pereira Mouriès, Lucilia and Capt, Annabelle and van Ree, Ronald
Journal: Clinical and translational allergy (2018): 30