AAT Bioquest


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Physical properties
Molecular weight692.77
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Coronaviruses (CoVs) can infect humans and multiple species of animals, causing a wide spectrum of diseases. In late 2019, a novel coronavirus, termed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was determined as a cause for several cases of respiratory disease (Covid-19). Even though most infected patients only suffer from mild symptoms such as fever and cough associated with a good prognosis, the disease can progress into fatal cases of pneumonia and acute respiratory failure, especially in older males with comorbidities. Covid-19 rapidly spread worldwide. As of May 31st, 2022, more than 6.2 million people have died from coronavirus worldwide, and ~530 million cases have been reported. Coronavirus is a single-stranded RNA positive-strand envelope type B coronavirus. Like the other two coronaviruses that cause SARS (Severe Acute Respiratory Syndrome) and MERS (Middle East Respiratory Syndrome), SARS-CoV-2 encodes non-structural, structural, and accessory proteins. Non-structural proteins include 3-chymotrypsin-like protease (3CLpro), papain-like protease, helicase, and RNA-dependent RNA polymerase (RNA -dependent RNA polymerase (RdRp). Structural proteins include spike glycoproteins. Papain in coronavirus operates on more than 11 cleavage sites on the large polyprotein 1ab. Processing of polyproteins translated from viral RNA is essential, therefore, the main proteases are identified as an attractive drug targets for preventing virus imitation. Papain-like protease (PLpro) of coronaviruses carries out proteolytic maturation of non-structural proteins that play a role in replication of the virus and performs deubiquitination of host cell factors to scuttle antiviral responses. Z-LRGG-AMC is cleaved by papain-like proteases to give the highly fluorescent AMC product. The fluorescence intensity of released AMC is proportional to the protease activity. Z-LRGG-AMC might be used for screening and studying kinetics of PLpro inhibitors.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Z-LRGG-AMC to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM144.348 µL721.74 µL1.443 mL7.217 mL14.435 mL
5 mM28.87 µL144.348 µL288.696 µL1.443 mL2.887 mL
10 mM14.435 µL72.174 µL144.348 µL721.74 µL1.443 mL

Molarity calculator

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Spectral properties

Excitation (nm)341
Emission (nm)441

Product Family

NameExcitation (nm)Emission (nm)



View all 50 references: Citation Explorer
Decoupling deISGylating and deubiquitinating activities of the MERS virus papain-like protease.
Authors: Clasman, Jozlyn R and Everett, Renata K and Srinivasan, Karthik and Mesecar, Andrew D
Journal: Antiviral research (2020): 104661
Characterization and noncovalent inhibition of the deubiquitinase and deISGylase activity of SARS-CoV-2 papain-like protease.
Authors: Freitas, Brendan T and Durie, Ian A and Murray, Jackelyn and Longo, Jaron E and Miller, Holden C and Crich, David and Hogan, Robert Jeff and Tripp, Ralph A and Pegan, Scott D
Journal: ACS infectious diseases (2020)
Structural and biochemical characterization of SADS-CoV papain-like protease 2.
Authors: Wang, Lu and Hu, Weihua and Fan, Chengpeng
Journal: Protein science : a publication of the Protein Society (2020): 1228-1241
First detection of novel enterovirus G recombining a torovirus papain-like protease gene associated with diarrhoea in swine in South Korea.
Authors: Lee, Sunhee and Lee, Changhee
Journal: Transboundary and emerging diseases (2019): 1023-1028
Identification and design of novel small molecule inhibitors against MERS-CoV papain-like protease via high-throughput screening and molecular modeling.
Authors: Lee, Hyun and Ren, Jinhong and Pesavento, Russell P and Ojeda, Isabel and Rice, Amy J and Lv, Haining and Kwon, Youngjin and Johnson, Michael E
Journal: Bioorganic & medicinal chemistry (2019): 1981-1989
Characterization of the Papain-Like Protease p29 of the Hypovirus CHV1-CN280 in Its Natural Host Fungus Cryphonectria parasitica and Nonhost Fungus Magnaporthe oryzae.
Authors: Xiong, Qin and Zhang, Linqiao and Waletich, Justin and Zhang, Linlin and Zhang, Chen and Zheng, Xinyue and Qian, Yulin and Zhang, Zhengguang and Wang, Yuanchao and Cheng, Qiang
Journal: Phytopathology (2019): 736-747
Analysis of Coronavirus Temperature-Sensitive Mutants Reveals an Interplay between the Macrodomain and Papain-Like Protease Impacting Replication and Pathogenesis.
Authors: Deng, Xufang and Mettelman, Robert C and O'Brien, Amornrat and Thompson, John A and O'Brien, Timothy E and Baker, Susan C
Journal: Journal of virology (2019)
The papain-like protease determines a virulence trait that varies among members of the SARS-coronavirus species.
Authors: Niemeyer, Daniela and Mösbauer, Kirstin and Klein, Eva M and Sieberg, Andrea and Mettelman, Robert C and Mielech, Anna M and Dijkman, Ronald and Baker, Susan C and Drosten, Christian and Müller, Marcel A
Journal: PLoS pathogens (2018): e1007296
Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibited by 6-thioguanine.
Authors: Chu, Hsu-Feng and Chen, Chiao-Che and Moses, David C and Chen, Yau-Hung and Lin, Chao-Hsiung and Tsai, Ying-Chieh and Chou, Chi-Yuan
Journal: Antiviral research (2018): 199-205
The papain-like protease of avian infectious bronchitis virus has deubiquitinating activity.
Authors: Yu, Liping and Zhang, Xiaorong and Wu, Tianqi and Wang, Yuyang and Meng, Jie and Liu, Qian and Niu, Xiaosai and Wu, Yantao
Journal: Archives of virology (2017): 1943-1950